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Cloning and expression analysis of human reticulon 4c cDNA

机译:人网状蛋白4c cDNA的克隆及表达分析

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摘要

RTNs (reticulons) is a gene family related to the growth and differentiation of neuroendocrine cell. This family is composed of several members such as RTN1, RTN2 and RTN3. RTN1 and RTN2 have been proved to have 3 transcripts with different length. Because the RTNc cDNA was involved in the sologenesis of mall cell lung carcinoma (SCLC), it was selected As a bioinformatic probe to clone novel members of RTN family with the electric hybridization Assistant gew-gene cloning method (EHAC). A 1677-bp cDNA was identified from human brain CDNA library.
机译:RTN(网状蛋白)是一个与神经内分泌细胞的生长和分化有关的基因家族。该家族由几个成员组成,例如RTN1,RTN2和RTN3。 RTN1和RTN2已被证明具有3个不同长度的转录本。由于RTNc cDNA参与了商城细胞肺癌(SCLC)的溶菌作用,因此选择它作为生物信息探针,通过电杂交助手gew基因克隆方法(EHAC)克隆RTN家族的新成员。从人脑CDNA文库中鉴定出1677 bp cDNA。

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