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Action mechanisms of a new erythrocyte-derived depression factor

机译:一种新的红细胞源性抑郁因子的作用机制

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To investigate the action mechanisms of a new erythrocyte-derived depressing factor (EDDF), the focus is placed on the effect of DEEF on both cytosolic and nuclear free calcium (Ca~2+) transportation in vascular smooth muscle cell (VSMC), as well as the apoptosis and cell cycle of VSMC of rats. EDDF has been extracted from human erythrocytes. The changes of Ca~2+ levels in cytoplasm ([Ca~2+]_I) and nucleus ([Ca~2+]_n) have been observed using a laser scanning confocal microscope together with fluo-3/AM as a calcium indicator.
机译:为了研究新的红细胞源性抑制因子(EDDF)的作用机理,重点研究了DEEF对血管平滑肌细胞(VSMC)中胞质和核游离钙(Ca〜2 +)转运的影响。以及大鼠血管平滑肌细胞的凋亡和细胞周期。 EDDF已从人红细胞中提取。激光扫描共聚焦显微镜结合fluo-3 / AM作为钙指示剂观察到细胞质([Ca〜2 +] _ I)和细胞核([Ca〜2 +] _ n)中Ca〜2 +水平的变化。

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