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Indentification and characterization of human neuronal voltage-gated calcium channel gamma 3 subunit gene

机译:人神经元电压门控钙通道γ3亚基基因的鉴定与表征

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摘要

By homologous expressed sequence tag (EST) searching, one EST (GenBank: W29095) was obtained, which shows 75/100 identity in 435 bp overlap with the conding sequence of mouse Cacng2 gene. A 1 545 bp cDNA fragment was obtained from the nested polymerase chain reaction (PCR) and rapid applification of cDNA end (RACE) reaction in the human brain prefrontal cortex cDNA library and the human brain Ready cDNA with the primers designed on W29095.
机译:通过同源表达序列标签(EST)搜索,获得了一个EST(GenBank:W29095),其在与小鼠Cacng2基因的编码序列重叠的435bp中显示出75/100的同一性。通过巢式聚合酶链反应(PCR)和人脑前额叶皮层cDNA文库和人脑Ready cDNA的快速末端cDNA末端反应(RACE),使用W29095设计的引物,获得了1 545 bp cDNA片段。

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