Novel carriers for nucleic acids subtractive hybridization: synthesis and hybridization efficiency

NI Xiaodong; LIU Dinggan; LI Zaiping

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Novel carriers for nucleic acids subtractive hybridization: synthesis and hybridization efficiency

机译：用于核酸减性杂交的新型载体：合成和杂交效率

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摘要

The molecular hybridization of nucleic acids is widely used in the detection and purification of specific sequences, especially for the isolation, purification and chromosomal localization of genes and their expression products. The subtractive hybridization, namely the hybridization for screening of the group-specific (differentially expressing) nucleic acids species from various tissues and cell lines, is a very important tool for the studies of tumor molecular biology and the tumor suppressor genes.

机译：核酸的分子杂交被广泛用于特定序列的检测和纯化，特别是基因及其表达产物的分离，纯化和染色体定位。消减杂交，即从各种组织和细胞系中筛选组特异性（差异表达）核酸物种的杂交，是研究肿瘤分子生物学和抑癌基因的非常重要的工具。

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来源
《Chinese science bulletin》 |1996年第14期|p.1207-1213|共7页
作者
NI Xiaodong; LIU Dinggan; LI Zaiping;
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作者单位

Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China;

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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
subtractive hybridization; SESA; glycogen; Sephadex;

机译：消减杂交;SESA;糖原;Sephadex;

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专利

1. Novel carriers for nucleic acids subtractive hybridization: synthesis and hybridization efficiency [J] . NI Xiaodong, LIU Dinggan, LI Zaiping Chinese science bulletin . 1996,第14期

机译：用于核酸减性杂交的新型载体：合成和杂交效率
2. Assessment of methods for covalent binding of nucleic acids to magnetic beads, DynabeadsTM, and the characteristics of the bound nucleic acids in hybridization reactions [J] . David Rickwood, Erik Homes, Federico Robbiati, Nucleic acids research . 1988,第22期

机译：评估核酸与磁珠，DynabeadsTM共价结合的方法以及杂交反应中结合核酸的特征
3. New Generation of Clickable Nucleic Acids: Synthesis and Active Hybridization with DNA [J] . Han Xun, Domaille Dylan W., Fairbanks Benjamin D., Biomacromolecules . 2018,第10期

机译：新一代可点击的核酸：用DNA合成和活性杂交
4. Chiral peptide nucleic acids:design,synthesis,optical purity and hybridization properties [C] . Stefano Sforza, Roberto Corradini, Tullia Tedeschi, European Peptide Symposium . 2002

机译：手性肽核酸：设计，合成，光学纯度和杂交性能
5. Synthetic studies on peptide nucleic acids: 1. Toward a circular bis-peptide nucleic acid. 2. Sequence and linker effects in bis-PNA hybridization to DNA. [D] . Tse, Joseph. 2004

机译：肽核酸的合成研究：1.形成环状双肽核酸。 2. bis-PNA与DNA杂交中的序列和接头效应。
6. Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes [O] . Kengo Kubota, Akiyoshi Ohashi, Hiroyuki Imachi, 2006

机译：锁定核酸整合DNA探针提高了原位杂交效率
7. Unlocked nucleic acids with a pyrene-modified uracil: Synthesis, hybridization studies, fluorescent properties and i-motif stability [O] . Perlíková P., Karlsen K.K., Pedersen E.B., 2014

机译：具有pyr修饰的尿嘧啶的未锁定核酸：合成，杂交研究，荧光性质和i-基序稳定性

Novel carriers for nucleic acids subtractive hybridization: synthesis and hybridization efficiency

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