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首页> 外文期刊>Chinese science bulletin >Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers
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Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers

机译:使用RAPD引物通过随机扩增的cDNA鉴定光周期敏感基因雄性不育水稻中的差异表达基因

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摘要

The fertile and sterile young panicle representational populations were constructed by bulked sampling method using young panicles in the photo-period sensitive stage of fertility transformation. Two populations were analyzed using cDNA-RAPD. The results showed that:(Ⅰ)bulked sampling Method can be employed to analyze differentially expressed genes using cDNA-RAPD, taking an advan- Tage in avoiding false positive caused by conventional sampling method.
机译:在生育力转化的光周期敏感阶段,采用幼穗通过大量采样的方法构建了可育和不育的幼穗代表性种群。使用cDNA-RAPD分析了两个种群。结果表明:(Ⅰ)采用批量采样法可利用cDNA-RAPD分析差异表达的基因,有利于避免常规采样法引起的假阳性。

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