Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter

Huang Guocun; Dong Yuemei; Sun Jingsan

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Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter

机译：以GFP为报告基因，通过花粉管途径将外源DNA导入棉花

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The green fluorescent protein(GFP)gene from the jellyfish Aequorea victoria as a vital reporter for gene expression in plants is considered to have several advantages over other reporter genes. The pBIN35S-mGFP4 plasmid DNA has been introduced into cotton embryos by the pollen-tube pathway method. A transformed seedling has been verified according to its GFP-related fluorescence and Southern blotting analysis. The results provided direct and con- Vincing facts in cytology and molecular biology for the pollen-tube pathway method, an efficient Transformation technique used in plants.

机译：来自水母维多利亚水母的绿色荧光蛋白（GFP）基因作为植物中基因表达的重要报告基因，被认为比其他报告基因具有多个优势。通过花粉管途径方法将pBIN35S-mGFP4质粒DNA引入棉花胚中。根据其与GFP相关的荧光和Southern印迹分析，已验证了转化的幼苗。该结果为花粉管途径方法（一种有效的植物转化技术）提供了细胞学和分子生物学方面的直接且令人信服的事实。

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《Chinese science bulletin》 |1999年第8期|p.698-701|共4页
作者
Huang Guocun; Dong Yuemei; Sun Jingsan;
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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
green fluorescent protein(GFP); pollen-tube pathway; cotton;

机译：绿色荧光蛋白（GFP）;花粉管途径棉;

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1. Detection of vector- and selectable marker-free transgenic maize with a linear GFP cassette transformation via the pollen-tube pathway [J] . Yang A, Su Q, An L, Journal of Biotechnology . 2009,第1期

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2. Secretion from bovine chromaffin cells acutely expressing exogenous proteins using a recombinant Semliki Forest virus containing an EGFP reporter. [J] . Knight DE Molecular and cellular neurosciences . 1999,第6期

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3. Recent Advances in GFP Folding Reporter and Split-GFP Solubility Reporter Technologies. Application to Improving the Folding and Solubility of Recalcitrant Proteins from Mycobacterium tuberculosis [J] . Stéphanie Cabantous, Jean-Denis Pédelacq, Brian L. Mark, Journal of structural and functional genomics . 2005,第2a3期

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4. High-throughput analysis of multiple stress pathways using GFP reporters in C. elegans [C] . D. de Pomerai, C. Anbalagan, I. Lafayette International Conference on Environmental Toxicology . 2010

机译：C.秀丽隐杆线虫的GFP记者使用GFP记者的高吞吐量分析
5. Development and Characterization of Caspase Activatable GFP and a Family of Fluorescent Reporters. [D] . Nicholls, Samantha B. 2012

机译：半胱天冬酶可激活的GFP和荧光记者家族的开发和表征。
6. A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway. [O] . D Ferrandon, A C Jung, M Criqui, 1998

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7. Introduction of a NIa proteinase cleavage site between the reporter gene and HC-Pro only partially restores the biological properties of GUS- or GFP-tagged LMV [O] . Sylvie German-Retana, Elise Redondo, Geneviève Tavert-Roudet, 2003

机译：在报告基因和HC-Pro之间引入NIA蛋白酶切割位点仅部分恢复GUS或GFP标记的LMV的生物学性质

Introduction of exogenous DNA into cotton via the pollen-tube pathway with GFP as a reporter

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