Generation of a recombinant herpes simplex virus which can provide packaging function for recombinant adeno-associated virus

Wu Zhijian; Wu Xiaobing; Hou Yunde

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Generation of a recombinant herpes simplex virus which can provide packaging function for recombinant adeno-associated virus

机译：可以为重组腺相关病毒提供包装功能的重组单纯疱疹病毒的产生

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The generation of a recombinant HSV(rHSV)that can provide packaging function for rAAV production is described. A set of cosmids including cos48, cos28, cos6, cos14 and cos56, which represents the HSV-1 genome was used for generation of this rHSV. Rep and cap genes of AAV-2 were inserted into XbaⅠsite of UL2 gene on cos6, generating cos6- rcΔUL2. After being digested with PacⅠ, cos6-rcΔUL2 and the other 4 cosmids were cotras- fected into BHK-21 cells. The recombinant virus HSV1-rc/ΔUL2 carrying rep cap genes was generated due to the homologous recombination of the 5 cosmids.

机译：描述了可为rAAV生产提供包装功能的重组HSV（rHSV）的产生。使用包括cos48，cos28，cos6，cos14和cos56在内的一组粘粒（代表HSV-1基因组）来生成该rHSV。将AAV-2的Rep和cap基因插入cos6上UL2基因的XbaⅠ位点，产生cos6-rcΔUL2。用PacⅠ消化后，将cos6-rcΔUL2和其他4种粘粒共转染到BHK-21细胞中。由于5个粘粒的同源重组，产生了带有rep cap基因的重组病毒HSV1-rc /ΔUL2。

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来源
《Chinese science bulletin》 |1999年第8期|p.715-719|共5页
作者
Wu Zhijian; Wu Xiaobing; Hou Yunde;
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原文格式 PDF
正文语种 eng
中图分类自然科学现状及发展;
关键词
recombinant AAV; packaging function; rcombinnant HSV;

机译：重组AAV;包装功能;重组HSV;

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专利

1. Preclinical evaluation of a recombinant adeno-associated virus vector expressing human alpha-1 antitrypsin made using a recombinant herpes simplex virus production method. [J] . Chulay JD, Ye GJ, Thomas DL, Human gene therapy . 2011,第2期

机译：用重组单纯疱疹病毒生产方法制备的表达人α-1抗胰蛋白酶的重组腺相关病毒载体的临床前评价。
2. Scalable Recombinant Adeno-Associated Virus Production Using Recombinant Herpes Simplex Virus Type 1 Coinfection of Suspension-Adapted Mammalian Cells [J] . Darby L. Thomas, Lijun Wang, Justine Niamke Human gene therapy . 2009,第8期

机译：悬浮适应哺乳动物细胞使用重组单纯疱疹病毒1型共感染的可扩展重组腺相关病毒生产。
3. High-titer recombinant adeno-associated virus production utilizing a recombinant herpes simplex virus type I vector expressing AAV-2 Rep and Cap. [J] . Conway JE, Rhys CM, Zolotukhin I, Gene therapy . 1999,第6期

机译：利用表达AAV-2 Rep和Cap的重组I型单纯疱疹病毒载体生产高滴度重组腺相关病毒。
4. Recombinant Adeno-associated virus (rAAV)-mediated transduction and optogenetic manipulation of cortical neurons in vitro [C] . Wienke Lange, Lei Jin, Vanessa Maybeck, Conference on Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics . 2014

机译：重组腺相关病毒（rAAV）介导的皮层神经元体外转导和光遗传学操作
5. Recombinant fowlpox-laryngotracheitis vaccine and recombinant herpes virus of turkey-laryngotracheitis vaccine induced immunity against infectious laryngotracheitis virus challenge. [D] . Luengo C., Amelia C. 1998

机译：重组鸡痘-喉气管炎疫苗和重组疱疹病毒-土耳其-喉气管炎疫苗可诱导抵抗传染性喉气管炎病毒攻击的免疫力。
6. Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and Cap. [O] . J E Conway, S Zolotukhin, N Muzyczka, 1997

机译：表达Rep和Cap的单纯疱疹病毒1型扩增子完全支持重组腺相关病毒2型复制和包装。
7. Preclinical Evaluation of a Recombinant Adeno-Associated Virus Vector Expressing Human Alpha-1 Antitrypsin Made Using a Recombinant Herpes Simplex Virus Production Method [O] . Chulay, Jeffrey D., Ye, Guo-Jie, Thomas, Darby L., 2011

机译：表达重组人单纯疱疹病毒生产方法表达人α-1抗胰蛋白酶的重组腺相关病毒载体的临床前评价

Generation of a recombinant herpes simplex virus which can provide packaging function for recombinant adeno-associated virus

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