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RAPD analysis of a new yellow rust resistant wheat-rye translocation line

机译：新型抗黄小麦黑麦易位系的RAPD分析

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Random amplified polymorphic DNAs (RAPD) analysis is a recently developed technique based on the polymerase chain reaction (PCR) . Usually, arbitrary decamer oligonucleotides are used in the PCR procedure at a low annealing temperature (e. g. 37 °C),thus correspondent DNA fragments can be amplified from several regions of the genome. RAPD analysis is relatively inexpensive and fast and needs less genomic DNA than the other methods as RFLP. Therefore, it is applicable to a wide range of research areas. Yellow rust is one of the most serious diseases of wheat in northern China. Particularly, with the appearance of the new yellow rust physiological races No.28 and No.29, the previous 1B/1R translocation lines such as Lovrins 10 and 13 are no longer used as resistant resources. The need of new varieties with a wider resistant spectrum is in emergency. We report here the analysis of such a new yellow rust resistant line derived from the translocation of wheat and rye chromosomes using RAPD analysis. The advantages and disadvantages of using RAPD in cereal genomic analysis are also discussed.
机译：随机扩增多态性DNA（RAPD）分析是基于聚合酶链反应（PCR）的最新开发技术。通常，在低温退火温度（例如37℃）下，在PCR过程中使用任意的十聚体寡核苷酸，因此可以从基因组的几个区域扩增相应的DNA片段。 RAPD分析相对便宜且快速，并且比其他方法（如RFLP）需要更少的基因组DNA。因此，它适用于广泛的研究领域。黄锈病是中国北方小麦最严重的病害之一。特别地，随着新的28号和29号黄锈病生理小种的出现，以前的1B / 1R易位系如Lovrins 10和13不再用作抗性资源。迫切需要具有更广泛抗药性的新品种。我们在这里报告了使用RAPD分析从小麦和黑麦染色体易位获得的这种新的抗黄锈新品系的分析。还讨论了在谷物基因组分析中使用RAPD的优缺点。

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