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首页> 外文期刊>Chromatographia >Influences of the mobile phase composition and temperature on the retention behavior of aromatic alcohol homologues in hydrophobic interaction chromatography
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Influences of the mobile phase composition and temperature on the retention behavior of aromatic alcohol homologues in hydrophobic interaction chromatography

机译：流动相组成和温度对疏水相互作用色谱中芳香醇同系物保留行为的影响

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摘要

To eliminate the very complicated effects of chromatographic thermodynamics in hydrophobic interaction chromatography (HIC) with biopolymers as solutes, homologues of neutral aromatic alcohols were selected as solutes for investigating their thermodynamic behavior in HIC. The effects of the mobile phase composition and temperature (0∼80°C) on the retention behavior of the homologues were studied extensively. The retention behavior of the homologue was characterized by the linear parameters in the stoichiometric displacement model for retention (SDM-R). The retention of small molecules is essentially controlled by non-specific interaction in HIC as well as reversed phase liquid chromatography (RPLC), and the parameters obtained were found to follow the homologue rule. Plots of the logarithm of retention of solutes in four kinds of salt solution versus the reciprocal of the absolute temperature over a wide range were nonlinear, indicating a large heat capacity change associated with retention. The thermodynamic parameters demonstrate the retention of small molecules in HIC to be entropy-driven at low temperature and enthalpy-driven at high temperature.
机译：为了消除以生物聚合物为溶质的疏水相互作用色谱（HIC）中色谱热力学的非常复杂的影响，选择中性芳族醇的同系物作为溶质，以研究其在HIC中的热力学行为。广泛研究了流动相组成和温度（0〜80℃）对同系物保留行为的影响。同源物的保留行为通过化学计量位移模型（SDM-R）中的线性参数来表征。小分子的保留基本上由HIC中的非特异性相互作用以及反相液相色谱（RPLC）控制，并且发现获得的参数遵循同源规则。四种盐溶液中溶质的保留率对数与绝对温度在宽范围内的倒数的关系图是非线性的，表明与保留率相关的热容变化很大。热力学参数表明，HIC中的小分子保留在低温下由熵驱动，而在高温下由焓驱动。

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