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首页> 外文期刊>Chromosoma >The enhancement of histone H4 and H2A serine 1 phosphorylation during mitosis and S-phase is evolutionarily conserved
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The enhancement of histone H4 and H2A serine 1 phosphorylation during mitosis and S-phase is evolutionarily conserved

机译：在进化上保守有丝分裂和S期期间组蛋白H4和H2A丝氨酸1磷酸化的增强。

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Histone phosphorylation has long been associated with condensed mitotic chromatin; however, the functional roles of these modifications are not yet understood. Histones H1 and H3 are highly phosphorylated from late G2 through telophase in many organisms, and have been implicated in chromatin condensation and sister chromatid segregation. However, mutational analyses in yeast and biochemical experiments with Xenopus extracts have demonstrated that phosphorylation of H1 and H3 is not essential for such processes. In this study, we investigated additional histone phosphorylation events that may have redundant functions to H1 and H3 phosphorylation during mitosis. We developed an antibody to H4 and H2A that are phosphorylated at their respective serine 1 (S1) residues and found that H4S1/H2AS1 are highly phosphorylated in the mitotic chromatin of worm, fly, and mammals. Mitotic H4/H2A phosphorylation has similar timing and localization as H3 phosphorylation, and closely correlates with the chromatin condensation events during mitosis. We also detected a lower level of H4/H2A phosphorylation in 5-bromo-2-deoxyuridine-positive S-phase cells, which corroborates earlier studies that identified H4S1 phosphorylation on newly synthesized histones during S-phase. The evolutionarily conserved phosphorylation of H4/H2A during the cell cycle suggests that they may have a dual purpose in chromatin condensation during mitosis and histone deposition during S-phase.
机译：组蛋白磷酸化长期以来一直与浓缩的有丝分裂染色质有关。但是，这些修改的功能作用尚不清楚。在许多生物中，组蛋白H1和H3从G2晚期到末期都高度磷酸化，并与染色质浓缩和姐妹染色单体分离有关。但是，酵母和非洲爪蟾提取物的生化实验中的突变分析表明，H1和H3的磷酸化对于此类过程不是必需的。在这项研究中，我们研究了额外的组蛋白磷酸化事件，这些事件可能对有丝分裂期间的H1和H3磷酸化具有多余的功能。我们开发了一种针对H4和H2A的抗体，这些抗体在其各自的丝氨酸1（S1）残基处被磷酸化，发现H4S1 / H2AS1在蠕虫，果蝇和哺乳动物的有丝分裂染色质中被高度磷酸化。有丝分裂的H4 / H2A磷酸化与H3磷酸化具有相似的时间和定位，并与有丝分裂期间的染色质浓缩事件密切相关。我们还检测到5-溴-2-脱氧尿苷阳性S期细胞中H4 / H2A磷酸化水平较低，这证实了较早的研究，该研究确定了S期期间新合成的组蛋白上的H4S1磷酸化。在细胞周期中，H4 / H2A的进化保守磷酸化表明，它们在有丝分裂期间的染色质浓缩和S期的组蛋白沉积中可能具有双重目的。

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  • 来源
    《Chromosoma》 |2004年第7期|360-371|共12页
  • 作者

    Cynthia M. Barber; Fiona B. Turner; Yanming Wang; Kirsten Hagstrom; Sean D. Taverna; Sahana Mollah; Beatrix Ueberheide; Barbara J. Meyer; Donald F. Hunt; Peter Cheung; C. David Allis;

  • 作者单位

    Department of Biochemistry and Molecular Genetics University of Virginia Health System;

    Department of Biochemistry and Molecular Genetics University of Virginia Health System;

    Laboratory of Chromatin Biology The Rockefeller University;

    Howard Hughes Medical Institute and Department of Molecular and Cell Biology University of California at Berkeley;

    Department of Biochemistry and Molecular Genetics University of Virginia Health SystemLaboratory of Chromatin Biology The Rockefeller University;

    Department of Chemistry University of Virginia;

    Department of Chemistry University of Virginia;

    Howard Hughes Medical Institute and Department of Molecular and Cell Biology University of California at Berkeley;

    Department of Chemistry University of Virginia;

    Department of Medical Biophysics Ontario Cancer Institute University of Toronto;

    Laboratory of Chromatin Biology The Rockefeller University;

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