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首页> 外文期刊>Clean >Kinetic Modeling for Simultaneous Biodegradation of Phenol and Formaldehyde at Inhibitory Concentrations by Ralstonia eutropha
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Kinetic Modeling for Simultaneous Biodegradation of Phenol and Formaldehyde at Inhibitory Concentrations by Ralstonia eutropha

机译：Ralstonia eutropha在抑制浓度下苯酚和甲醛同时生物降解的动力学模型

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摘要

The degradation of phenol and formaldehyde (FA) at different concentrations as single or binary systems were experimentally investigated by a pure culture of Ralstonia eutropha in a batch mixed bioreactor operated at 30°C, initial pH 6.5, an aeration rate of 0.5 vvm, and 200 rpm, to determine the kinetics parameters of the processes. In single substrate experiments, the results of the kinetics studies with the Andrews and Noack equation showed that phenol and FA had a self-inhibitory effect on their biodegradation when the initial concentration was higher than 284.1 and 215.4mgL_1, respectively. In addition, kinetics studies indicated that partial non-competitive enzyme inhibition could be used to describe the interactional effects of phenol and FA in the simultaneous biodegradation process. According to the non-competitive inhibition kinetics applied, the inhibitory constant of phenol on FA biodegradation (KI 1!2) and partiality factor (b1!2) were estimated to be 81.31mgL_1 and 0.357. In order to describe the effect of FA concentration on the phenol biodegradation, the following results were obtained: KI 2!1¼37.63 and b2!1¼0.171mgL_1. The prediction capacity of the estimated parameters was examined using a model which could describe the dynamic feature of the bioprocesses.
机译：通过在30°C，初始pH 6.5，曝气速率为0.5 vvm和200 rpm，以确定过程的动力学参数。在单底物实验中，使用Andrews和Noack方程进行的动力学研究结果表明，当初始浓度分别高于284.1和215.4mgL_1时，苯酚和FA对它们的生物降解具有自抑制作用。此外，动力学研究表明，部分非竞争性酶抑制作用可用于描述酚和FA在同时生物降解过程中的相互作用。根据所应用的非竞争性抑制动力学，苯酚对FA生物降解的抑制常数（KI 1！2）和偏倚因子（b1！2）估计为81.31mgL_1和0.357。为了描述FA浓度对苯酚生物降解的影响，获得了以下结果：KI 2！1¼37.63和b2！1¼0.171mgL_1。使用可以描述生物过程动态特征的模型检查了估计参数的预测能力。

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