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首页> 外文期刊>Clinical Laboratory >Immunonephelometric Determination of Fibrinogen on Citrated or Heparinized Plasma: Comparison with Functional Clauss Method
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Immunonephelometric Determination of Fibrinogen on Citrated or Heparinized Plasma: Comparison with Functional Clauss Method

机译：柠檬酸化或肝素化血浆中免疫纤维蛋白原测定：与功能条款法的比较

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摘要

Background: Fibrinogen is routinely determined by functional assay on citrated plasma in the hematology department. However, immunoassay can be performed easily with nephelometric analyzer in the clinical chemistry laboratory allowing automatization. The aim of this study was first to compare the clotting von Clauss method (activity assay) with an immunonephelometric method (antigen assay) on the BN ProSpec™ (Dade Behring). Moreover, we evaluated the possibility of collecting blood samples on heparin to facilitate blood collection for clinicians and reduce required blood collection volumes for dosages. Methods: In a first step of experiment, the accuracy of immunonephelometric analytical performance was tested on heparinized and citrated tubes. For comparison studies, fibrinogen activity was then determined on citrated tubes in the hematology department and antigen measurement was performed on both citrated and heparinized plasma from 130 consecutive patients. Results: As a result, the immunonephelometric method shows reliable performance and clinical sample measurements are not affected by the method used, validating the use of heparinized plasma samples for fibrinogen antigen determination with Dade Behring reagents.
机译：背景：纤维蛋白原通常通过血液学部门在柠檬酸盐血浆上进行功能测定来确定。但是，可以使用临床化学实验室中的浊度分析仪轻松进行免疫分析，从而实现自动化。这项研究的目的是首先比较BN ProSpec™（Dade Behring）上的凝血von Clauss方法（活性测定）与免疫比浊法（抗原测定）。此外，我们评估了在肝素上收集血样以方便临床医生采血并减少所需剂量的采血量的可能性。方法：在实验的第一步中，在肝素化和柠檬酸化的试管上测试免疫浊度分析性能的准确性。为了进行比较研究，随后在血液科确定了柠檬酸盐管上的纤维蛋白原活性，并对来自130位连续患者的柠檬酸盐和肝素化血浆进行了抗原测定。结果：结果，免疫比浊法显示出可靠的性能，临床样品的测量不受所用方法的影响，从而验证了肝素化血浆样品与Dade Behring试剂一起用于血纤蛋白原抗原测定的有效性。

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