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首页> 外文期刊>Combinatorial Chemistry & High Throughput Screening >Gel-Based Versus Gel-Free Proteomics: A Review
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Gel-Based Versus Gel-Free Proteomics: A Review

机译：基于凝胶与无凝胶蛋白质组学：综述

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摘要

With the sequencing of the genome of over 150 organisms, the field of biology has beennrevolutionised. Instead of studying one gene or protein at the time, it is now possible to study the effect ofnphysiological or pathological changes on the expressioin of all genes or proteins in the organism. Proteomicsnaims at the simultaneous analysis of all proteins expressed by a cell, tissue or organism in a specificnphysiological condition. Because proteins are the effector molecules in all organsims, it is evident thatnchanges in the physiological condition of an organism will be reflected by changes in protein expressionnand/or processing. Since the formulation of the concept of proteomics in the mid 90’s proteomics has reliednheavily on 2 dimensional gel electroforesis (2DGE) for the separation and visualization of proteins. 2DGE,nhowever, has a number of inherent drawbacks. 2DGE is costly, fairly insensitive to low copy proteins andncannot be used for the entire proteome. Therefore, over the years, several gel-free proteomics techniques havenbeen developed to either fill the gaps left by 2DGE or to entirely abolish the gel based techniques. This reviewnsummarizes the most important gel-free and gel-based proteomics techniques and compares their advantagesnand drawbacks.
机译：通过对150多种生物的基因组进行测序，生物学领域已经发生了革命性的变化。现在可以代替研究一个基因或蛋白质，而是可以研究生理或病理变化对生物中所有基因或蛋白质表达的影响。蛋白质组学旨在同时分析细胞，组织或生物体在特定生理条件下表达的所有蛋白质。因为蛋白质是所有器官中的效应分子，所以很明显，生物体生理状况的变化将由蛋白质表达和/或加工的变化反映出来。自从90年代中期提出蛋白质组学概念以来，蛋白质组学就大大依赖于二维凝胶电泳（2DGE）来分离和可视化蛋白质。但是2DGE具有许多固有的缺点。 2DGE价格昂贵，对低拷贝蛋白质相当不敏感，因此无法用于整个蛋白质组。因此，多年来，已经开发了几种无凝胶蛋白组学技术来填补2DGE留下的空白或完全废除基于凝胶的技术。本文综述了最重要的无凝胶和基于凝胶的蛋白质组学技术，并比较了它们的优缺点。

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