Combinatorial Libraries of Biocatalysts: Application and Screening

Laura Cipolla

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Combinatorial Libraries of Biocatalysts: Application and Screening

机译：生物催化剂组合库：应用和筛选

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Enzymes can perform intricate regioselective and / or enantioselective chemical transformations and can accelerate reaction rates by enormous factors all under mild conditions. However, enzymes almost always present problems for use on an industrial scale. Evolutionary design approaches can be applied to the generation of stable enzymes with improved or novel catalytic activities. Directed evolution can be considered as the biotechnological equivalent of combinatorial chemistry, where the expressed proteins are the combinatorial libraries of biocatalysts. This review will focus on the search of novel biocatalysts produced by genetic engineering with modified activity and stability in different environments, substrate specificity and enantioselectivity. Methods of screening and / or selection for the desired properties will also be described. Finally, the efforts in de novo enzyme design are mentioned.

机译：酶可以执行复杂的区域选择性和/或对映选择性化学转化，并且可以在温和条件下通过巨大的因素来加快反应速度。但是，酶几乎总是在工业规模上存在问题。进化设计方法可用于产生具有改进或新颖催化活性的稳定酶。定向进化可视为组合化学的生物技术等效物，其中表达的蛋白质是生物催化剂的组合文库。这项审查将侧重于由遗传工程生产的新型生物催化剂的搜索，这些催化剂在不同环境中具有改进的活性和稳定性，底物特异性和对映选择性。还将描述筛选和/或选择所需特性的方法。最后，提到了从头酶设计的努力。

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《Combinatorial Chemistry & High Throughput Screening》 |2004年第2期|p.101-114|共14页
作者
Laura Cipolla;
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作者单位

Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.za dellaScienza 2, I-20126 Milano, Italy.;

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正文语种 eng
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关键词
Biocatalysts, enzyme, enantioselective, catalytic, genetic engineering;

机译：生物催化剂;酶;对映选择性;催化;基因工程;

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1. Combinatorial libraries of biocatalysts: application and screening. [J] . Cipolla L Combinatorial chemistry & high throughput screening . 2004,第2期

机译：生物催化剂组合库：应用和筛选。
2. Phage-displayed combinatorial peptide libraries in fusion to beta-lactamase as reporter for an accelerated clone screening: Potential uses of selected enzyme-linked affinity reagents in downstream applications. [J] . Shukla GS, Krag DN Combinatorial chemistry & high throughput screening . 2010,第1期

机译：与β-内酰胺酶融合的噬菌体展示组合肽库，可作为报告基因用于加速克隆筛选：下游应用中潜在的酶联亲和试剂的潜在用途。
3. Applications of random sampling to virtual screening of combinatorial libraries [J] . Paul Beroza, Erin K. Bradley, John E. Eksterowicz, Journal of molecular graphics & modelling . 2000,第4a5期

机译：随机抽样在组合库虚拟筛选中的应用
4. Combinatorial Screening of Yeast Displayed Libraries Using Yeast Displayed Targets [C] . Kaitlyn Bacon, Matthew Burroughs, Stefano Menegatti, Protein Engineering Summit. . 2018

机译：使用酵母显示酵母的组合筛选酵母显示的图书馆
5. Screening combinatorial peptide libraries in complex mixtures for applications in therapeutic delivery and molecular diagnostics. [D] . Hall, Sejal Sampat. 2008

机译：筛选复杂混合物中的组合肽库，以用于治疗性递送和分子诊断。
6. Phage-displayed combinatorial peptide libraries in fusion to β-lactamase as reporter for an accelerated clone screening: Potential uses of selected enzyme-linked affinity reagents in downstream applications [O] . Girja S. Shukla, David N. Krag -1

机译：噬菌体展示的组合肽文库融合到β-内酰胺酶作为加速克隆筛选的记者：在下游应用中所选酶联亲和试剂的潜在用途
7. Combinatorial references Combinatorial libraries: Synthesis, screening and application potential edited by Riccardo Cortese, Walter de Gruyter, Berlin and New York, 1996. 232 pp. $102.25 (hardcover) ISBN3-11-014395-X [O] . Famulok Michael 1996

机译：组合参考书组合库：合成，筛选和应用潜力，由Riccardo Cortese，Walter de Gruyter，柏林和纽约编辑，1996年。232页，$ 102.25（精装）ISBN3-11-014395-X
8. Developing New Tools for the in vivo Generation/Screening of Cyclic Peptide Libraries. A New Combinatorial Approach for the Detection of Bacterial Toxin Inhibitors [R] . Camarero, J. A. 2007

机译：开发用于体内生成/筛选环肽库的新工具。用于检测细菌毒素抑制剂的新组合方法

Combinatorial Libraries of Biocatalysts: Application and Screening

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