Genotype-Phenotype Linkage for Directed Evolution and Screening of Combinatorial Protein Libraries

N. Doi H. Yanagawa

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Genotype-Phenotype Linkage for Directed Evolution and Screening of Combinatorial Protein Libraries

机译：基因型-表型连锁的定向进化和组合蛋白文库的筛选。

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摘要

The technologies for screening peptide and protein libraries for studies in the fields of directed protein evolution and functional genomics have advanced with astonishing speed. For screening of functional proteins, three technologies are required: (i) the construction of a gene library (genotype), (ii) the establish-ment of a linkage between each protein (phenotype) and its encoding gene (genotype), and (iii) the selection of desired proteins (phenotype) from the library. This review highlights the genotype-phenotype linkage technologies, which can be classified into three types that is, cell-type linkage, virus-type linkage, and array-type linkage methods. These methods are summarized, and their advantages and disadvantages are discussed.

机译：筛选肽和蛋白质文库以进行定向蛋白质进化和功能基因组学研究的技术以惊人的速度发展。为了筛选功能蛋白，需要三种技术：（i）构建基因文库（基因型），（ii）建立每种蛋白（表型）与其编码基因（基因型）之间的连接，以及（ iii）从文库中选择所需蛋白质（表型）。这篇综述重点介绍了基因型-表型连锁技术，可以将其分为三种类型，即细胞型连锁，病毒型连锁和阵列型连锁。总结了这些方法，并讨论了它们的优缺点。

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来源
《Combinatorial Chemistry & High Throughput Screening》 |2001年第6期|p.497-509|共13页
作者
N. Doi H. Yanagawa;
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作者单位

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kouhoku-Ku, Yokohama 223-8522, Japan;

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原文格式 PDF
正文语种 eng
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关键词
peptide, protein libraries, gene library, protein phenotype, gennotype phenotype linkage, cell type linkage, virus type linkage, array type linkage, polymerase chain reaction, pcr based mutagenesis, genotype linkage;

机译：肽;蛋白质文库;基因文库;蛋白质表型;基因型表型连锁;细胞型连锁;病毒型连锁;阵列型连锁;聚合酶链反应;基于pcr的诱变;基因型连锁;

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专利

1. New genotype-phenotype linkages for directed evolution of functional proteins [J] . Leemhuis H, Stein V, Griffiths AD, Current Opinion in Structural Biology . 2005,第4期

机译：用于功能蛋白定向进化的新基因型-表型连接
2. Front Cover: Beating Bias in the Directed Evolution of Proteins: Combining High‐Fidelity on‐Chip Solid‐Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction (ChemBioChem 3/2018) [J] . Li Aitao, Acevedo‐Rocha Carlos G., Sun Zhoutong, Chembiochem: A European journal of chemical biology . 2018,第3期

机译：前盖：在蛋白质的定向演变中击败偏见：将高保真片上的固相基因合成与有效基因组合组合中的组合图书馆建设（ChemBiochem 3/2018）
3. Beating Bias in the Directed Evolution of Proteins: Combining High‐Fidelity on‐Chip Solid‐Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction [J] . Li Aitao, Acevedo‐Rocha Carlos G., Sun Zhoutong, Chembiochem: A European journal of chemical biology . 2018,第3期

机译：在蛋白质的定向演变中击败偏见：将高保真片上固相基因合成与有效基因组合组合，用于组合图书馆建设
4. Identification of Inhibitors of Protein-Protein Interactions Through the Screening of Simplified Combinatorial Peptide Libraries [C] . Pasqualina L. Scognamiglio, Nunzianna Doti, Carlo Pedone American Peptide Symposium . 2009

机译：通过筛选简化组合肽文库鉴定蛋白质 - 蛋白质相互作用的抑制剂
5. In vitro selection to create new protein libraries and directed evolution of new ATP-binding proteins from a zinc finger scaffold usingmRNA display. [D] . Cho, Glen S. 2004

机译：使用mRNA展示从锌指支架中进行体外选择以创建新的蛋白质文库并指导新的ATP结合蛋白的进化。
6. Correction for Wu et al. Machine learning-assisted directed protein evolution with combinatorial libraries [O] . 2020

机译：对Wu等人的修正结合组合文库的机器学习辅助的定向蛋白质进化
7. Front Cover: Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction (ChemBioChem 3/2018) [O] . Aitao Li, Carlos G. Acevedo-Rocha, Zhoutong Sun, 2018

机译：前盖：在蛋白质的定向演变中击败偏见：将高保真片上的固相基因合成与有效基因组合组合中的组合图书馆建设（ChemBiochem 3/2018）

Genotype-Phenotype Linkage for Directed Evolution and Screening of Combinatorial Protein Libraries

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