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首页> 外文期刊>Composites >In vitro bioactivity and cytocompatablity of an injectable calcium phosphate cement/silanated gelatin microsphere composite bone cement
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In vitro bioactivity and cytocompatablity of an injectable calcium phosphate cement/silanated gelatin microsphere composite bone cement

机译：注射用磷酸钙水泥/硅烷化明胶微球复合骨水泥的体外生物活性和细胞相容性

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Different weight ratios of microemulsion-based gelatin uniform microspheres (GMs) were added to the tetra calcium phosphate (TTCP)-based cement (CPC) to investigate the physical, mechanical and apatite-forming ability of the CPC. The GMs contained 20 wt% of glycidoxypropyltrimethoxysilane (EPPTMS) as a crosslinking agent. The average size of GMs was on the order of 35 mu m. For CPC, the initial and final setting times were 10 min and 21 min, respectively. These values were prolonged up to 18 min and 28 min, respectively, for CPC containing 10 wt% of GM (CPC-10GM). The highest injectability percentage and injection time was related to the CPC-10GM. The highest mechanical values were related to CPC-5GM while CPC-10GM possessed the lowest compressive strength and Young's modulus. Apatite formation on the surfaces of all samples was confirmed by acellular in vitro bioactivity experiment after various periods of soaking time in simulated body fluid (SBF). Needle-like HA precipitates were manifested on the surface of CPC-5GM. According to MTT colorimetric assay, the viability rate of cells on the surfaces of CPC-5GM and CPC increases significantly with time. An appropriate attachment of G-292 cells on the surface of both samples after 48 h was observed by DAPI staining and SEM imaging methods. There was considerable difference in the ALP activity per cell basis between CPC and CPC-5GM at intervals of 7-21 days. These results indicate the potential of the CPC-5GM composites to be used in bone replacement.
机译：将不同重量比的微乳液基明胶均匀微球（GMs）添加到磷酸四钙（TTCP）基水泥（CPC）中，以研究CPC的物理，机械和磷灰石形成能力。 GM包含20重量％的环氧丙氧基丙基三甲氧基硅烷（EPPTMS）作为交联剂。转基因食品的平均大小约为35微米。对于CPC，初始设置时间和最终设置时间分别为10分钟和21分钟。对于含有10 wt％GM的CPC（CPC-10GM），这些值分别延长到18分钟和28分钟。最高可注射百分比和注射时间与CPC-10GM有关。最高机械值与CPC-5GM有关，而CPC-10GM具有最低的抗压强度和杨氏模量。在模拟体液（SBF）中浸泡了不同时间后，通过无细胞体外生物活性实验确认了所有样品表面的磷灰石形成。 CPC-5GM的表面出现针状HA沉淀物。根据MTT比色法，CPC-5GM和CPC表面细胞的存活率随时间显着增加。通过DAPI染色和SEM成像方法观察到48 h后G-292细胞在两个样品表面的适当附着。 CPC和CPC-5GM之间的间隔为7-21天时，每个细胞的ALP活性存在显着差异。这些结果表明CPC-5GM复合材料在骨替代中的潜力。

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