Genetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNA

Danillo Pinhal; Otto B. F. Gadig; Cesar Martins

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Genetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNA

机译：利用PCR-RFLP和5S rDNA核苷酸序列分析对鲨鱼Rhozoprionodon porosus和R. lalandii进行遗传鉴定

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A molecular approach based on nuclear 5S rDNA sequence variability was applied successfully to correctly identify samples from the two Rhizoprionodon species collected in the wild or sold in markets. The sequence of the non-transcribed spacer (NTS) of the 5S rDNA showed high interspecific variability and no intraspecific polymorphism, making it a useful marker for sharpnose shark identification. Polymorphisms in the NTS sequences of Rhizoprionodon sharks also created unique restriction patterns for each species after PCR-RFLP analysis. The 5S rDNA polymorphism represents a fast and non expensive tool to access species identification when rapid and unequivocal identification of shark products is needed, particularly for future management and other investigations.

机译：成功地应用了基于核5S rDNA序列变异性的分子方法，从野生或收集的两种根瘤菌物种中正确鉴定出样品。 5S rDNA的非转录间隔区（NTS）的序列显示出高的种间变异性，并且没有种内多态性，使其成为识别尖锐鲨鱼的有用标记。 PCR-RFLP分析后，根瘤菌鲨NTS序列的多态性也为每个物种创建了独特的限制性模式。 5S rDNA多态性代表了一种快速且不昂贵的工具，可用于需要快速，明确地鉴定鲨鱼产品的物种识别，尤其是在将来的管理和其他研究中。

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来源
《Conservation Genetics Resources》 |2009年第1期|35-38|共4页
作者
Danillo Pinhal; Otto B. F. Gadig; Cesar Martins;
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作者单位

Departamento de Morfologia Instituto de Biociências UNESP—Universidade Estadual Paulista Botucatu SP CEP 18618-000 Brazil;

Campus Litoral Paulista UNESP—Universidade Estadual Paulista São Vicente SP Brazil;

Departamento de Morfologia Instituto de Biociências UNESP—Universidade Estadual Paulista Botucatu SP CEP 18618-000 Brazil;

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正文语种 eng
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关键词
Genetic markers; Non-transcribed spacer; Trade monitoring; Sharks; Species identification;

机译：遗传标记;非转录间隔子;贸易监测;鲨鱼;物种鉴定;

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1. Identification of the shark species Rhizoprionodon lalandii and R-porosus (Elasmobranchii, Carcharhinidae) by multiplex PCR and PCR-RFLP techniques [J] . Mendonca FF, Hashimoto DT, Porto-Foresti F, Molecular ecology resources . 2009,第3期

机译：多重PCR和PCR-RFLP技术鉴定鲨鱼Rhizoprionodon lalandii和R-porosus（Ebramobranchii，Carcharhinidae）
2. Molecular organization of 5S rDNA in sharks of the genus Rhizoprionodon: insights into the evolutionary dynamics of 5S rDNA in vertebrate genomes [J] . Pinhal D, Araki CS, Gadig OB, Genetical Research . 2009,第1期

机译：根瘤菌鲨中5S rDNA的分子组织：脊椎动物基因组中5S rDNA进化动力学的见解。
3. ASPECTOS BIOLóGICOS DE RHIZOPRIONODON LALANDII Y RHIZOPRIONODON POROSUS (CARCHARHINIDAE-CARCHARHINIFORMES) CAPTURADOS MEDIANTE LA PESCA ARTESANAL EN ISLA FUERTE, CARIBE COLOMBIANO* [J] . Martínez Dallos Iván, Neira álvarez Mayerly, Acero P. Arturo Boletín de Investigaciones Marinas y Costeras - INVEMAR . 2012,第1期

机译：在哥伦比亚加勒比海的伊斯拉富埃特手工捕捞捕获的根瘤菌（Rhizoprionodon lalandii）和根瘤菌（rhizopriondon）的生物学方面*
4. Phylogenetic analyses based on ITS regions of rDNA identified five Rhizopus strains from tempeh as R. delemar and R. oryzae [C] . M. Khasanah, W. Sjamsuridzal, A. Oetari, International Symposium on Current Progress in Mathematics and Sciences . 2018

机译：基于RDNA地区的系统发育分析鉴定了来自Tempeh的五种Rhizopus菌株作为R. delemar和R. Oryzae
5. Identification and phylogenetic analysis of southeastern United States earthworm species using 16S rDNA and CO1 sequences. [D] . Leontieva, Yulia A. 2007

机译：使用16S rDNA和CO1序列鉴定和鉴定美国东南worm物种。
6. The 5S rDNA of the bivalve Cerastoderma edule: nucleotide sequence of the repeat unit and chromosomal location relative to 18S–28S rDNA [O] . Ana Insua, Ruth Freire, Josefina Méndez 1999

机译：双壳硬皮病皮肤的5S rDNA：相对于18S–28S rDNA的重复单元核苷酸序列和染色体位置
7. Use of single-stranded conformational polymorphisms (SSCP) to detect species relationship and population structure in the Atlantic sharpnose shark (Rhizoprionodon terraenovae) and the Caribbean sharpnose shark (R. porosus); Uso de los polimorfismos solo-trenzados de conformational (SSCPs) de detectar la relacion de la especie y la estructura de la poblacion en los de Rhizoprionodon terraenovae y R. porosus [O] . Todd T.N., Waldbeser L.S., Ward R. 2004

机译：利用单链构象多态性（SSCP）检测大西洋尖嘴鲨（Rhizoprionodon terraenovae）和加勒比尖嘴鲨（R. porosus）的物种关系和种群结构；利用构象单链多态性（SSCPs）来检测陆地根霉（Rhizoopionodon terraenovae）和孔雀（R. porosus）物种与种群结构的关系

Genetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNA

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