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首页> 外文期刊>Coordination chemistry reviews >Building from the 'Ground' Up: Developing interfacial chemistry for solid-phase nucleic acid hybridization assays based on quantum dots and fluorescence resonance energy transfer
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Building from the 'Ground' Up: Developing interfacial chemistry for solid-phase nucleic acid hybridization assays based on quantum dots and fluorescence resonance energy transfer

机译:从“地面”向上构建:为基于量子点和荧光共振能量转移的固相核酸杂交测定开发界面化学

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摘要

Improved diagnostic methods for the detection of specific nucleic acid biomarkers can potentially provide solutions to current challenges in healthcare, public safety, and environmental assessment. Here, we review the application of semiconductor quantum dots (QDs) in the development of nucleic acid hybridization assays that operate on the basis of fluorescence resonance energy transfer (FRET). Within the context of other important contributions to the field, we provide an account of our recent efforts and results toward multiplexed solid-phase hybridization assays. Emphasis is placed on interfacial chemistry, including the use of surface ligands and biomolecular tethers to immobilize QDs on a variety of solid substrates; factors affecting the non-specific adsorption of oligonucleotides on QDs; and different strategies for the biofunctionalization of immobilized QDs. The impact of interfacial chemistry on assay figures of merit such as sensitivity, selectivity, speed, or potential for regeneration is also considered. Further attention is paid to the design of FRET configurations that permit spectrally multiplexed detection of target nucleic acid sequences. Given that the intent of this review is to provide a consolidated framework upon which further advances can be made, we suggest some directions and challenges for future work.
机译:用于检测特定核酸生物标记物的改进诊断方法可以潜在地提供解决方案,以应对医疗保健,公共安全和环境评估方面的当前挑战。在这里,我们回顾了半导体量子点(QDs)在基于荧光共振能量转移(FRET)的核酸杂交检测技术的发展中的应用。在对该领域的其他重要贡献的背景下,我们提供了我们对多元固相杂交测定的最新努力和结果。重点放在界面化学上,包括使用表面配体和生物分子系链将QD固定在各种固体基质上;影响寡核苷酸在量子点上非特异性吸附的因素;以及固定化QDs生物功能化的不同策略。还考虑了界面化学对测定品质因数(例如灵敏度,选择性,速度或再生潜力)的影响。进一步关注FRET配置的设计,该配置可对目标核酸序列进行光谱多重检测。鉴于这次审查的目的是提供一个可以进一步取得进展的统一框架,我们为今后的工作提出了一些方向和挑战。

著录项

  • 来源
    《Coordination chemistry reviews》 |2014年第3期|25-52|共28页
  • 作者单位

    Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North,Mississauga, ON, Canada L5L 1C6;

    Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada VST 1Z1;

    Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North,Mississauga, ON, Canada L5L 1C6;

    Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North,Mississauga, ON, Canada L5L 1C6;

    Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, BC, Canada VST 1Z1;

    Chemical Sensors Group, Department of Chemical and Physical Sciences, University of Toronto Mississauga, 3359 Mississauga Road North,Mississauga, ON, Canada L5L 1C6;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Quantum dot; Fluorescence resonance energy transfer; Assay; Oligonucleotide; Immobilization; Adsorption;

    机译:量子点荧光共振能量转移;含量;寡核苷酸;固定;吸附性;

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