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首页> 外文期刊>Crop and Pasture Science >RAPD-PCR identification of Verticillium dahliae isolates with differential pathogenicity on cotton
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RAPD-PCR identification of Verticillium dahliae isolates with differential pathogenicity on cotton

机译：棉花黄萎病致病性不同的黄萎病菌的RAPD-PCR鉴定

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Strains of the fungal wilt pathogen Verticillium dahliae cause considerable economic losses in field crops throughout the world. We have investigated the use of molecular genetic techniques for the identification and classification of strains infecting cotton plants (Gossypium spp.) in the major production regions of Australia. The amplification, sequencing, and restriction digestion of specific ribosomal DNA sequences proved to be diagnostic for the genus/species, but was incapable of differentiating between individual isolates with quite diverse morphologies or origins. RAPD-PCR analysis using a subset of 13 informative decamer primers, however, did reveal significant differences between the isolates and enabled the genetic similarity of the V. dahliae strains to be estimated. RAPD-PCR fingerprints were shown to be reproducible following in planta culture of V. dahliae isolates and could be a valuable tool for the identification and epidemiological study of fungal populations. Although fungal isolates were found to elicit a range of reactions during pathogenicity testing on 5 cotton cultivars, comparison of the RAPD-PCR banding patterns did not suggest a strong correlation between molecular genetic and virulence characteristics.
机译：真菌枯萎病原菌黄萎病菌在全世界的田间作物中造成相当大的经济损失。我们已经研究了分子遗传技术在澳大利亚主要产区的感染棉花植物（棉）的菌株的鉴定和分类。特定核糖体DNA序列的扩增，测序和限制性消化被证明可诊断属/种，但无法区分形态或来源各异的分离株。然而，使用13种信息丰富的十聚体引物的子集进行的RAPD-PCR分析确实揭示了分离物之间的显着差异，并能够估算大丽弧菌菌株的遗传相似性。 RAPD-PCR指纹图谱显示在大疱弧菌分离株的植物培养后具有可重现性，并且可能是鉴定和进行真菌种群流行病学研究的宝贵工具。尽管在5个棉花品种的致病性测试过程中发现了真菌分离株，引发了一系列反应，但RAPD-PCR条带模式的比较并未表明分子遗传和毒力特性之间存在很强的相关性。

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