...
首页> 外文期刊>Current Genetics >Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria
【24h】

Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria

机译:肠菌根介导的鹅膏菌高亲和力铵导入基因AmAMT2的抑制。

获取原文
获取原文并翻译 | 示例
           

摘要

A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was identified in an EST project of functional Amanita muscaria/poplar ectomycorrhizas. Heterologous expression of the entire AmAMT2 coding region in yeast revealed the corresponding protein to be a high-affinity ammonium importer. In axenically grown Amanita hyphae AmAMT2 expression was strongly repressed by nitrogen, independent of whether the offered nitrogen source was transported by AmAMT2 or not. In functional ectomycorrhizas the AmAMT2 transcript level was further decreased in both hyphal networks (sheath and Hartig net), while extraradical hyphae revealed strong gene expression. Together our data suggest that (1) AmAMT2 expression is regulated by the endogenous nitrogen content of hyphae and (2) fungal hyphae in ectomycorrhizas are well supported with nitrogen even when the extraradical mycelium is nitrogen limited. As a consequence of AmAMT2 repression in mycorrhizas, ammonium can be suggested as a potential nitrogen source delivered by fungal hyphae in symbiosis.
机译:外生菌根的主要功能是某些土壤真菌与木本植物细根之间的共生,是将植物来源的碳水化合物交换为真菌来源的营养素。由于需要大量氮,因此特别感兴趣。在功能性鹅膏菌/杨树外生菌根的EST项目中确定了一个编码推定的真菌铵输入物的基因(AmAMT2)。酵母中整个AmAMT2编码区的异源表达表明相应的蛋白质是高亲和力的铵输入物。在无菌生长的鹅膏菌菌丝中,AmAMT2的表达受到氮的强烈抑制,与提供的氮源是否由AmAMT2转运无关。在功能性外生菌根中,两个菌丝网络(鞘和Hartig网)的AmAMT2转录水平进一步降低,而根外菌丝则显示出强大的基因表达。我们的数据共同表明,(1)AmAMT2表达受菌丝的内源性氮含量调节,(2)即使根外菌丝体受氮限制,外生菌根中的真菌菌丝也得​​到了很好的氮支持。菌根中AmAMT2抑制的结果是,铵盐可能被认为是共生中真菌菌丝传递的潜在氮源。

著录项

  • 来源
    《Current Genetics》 |2007年第2期|71-78|共8页
  • 作者单位

    Physiologische Ökologie der Pflanzen Eberhard Karls Universität Auf der Morgenstelle 1 72076 Tübingen Germany;

    Spezielle Botanik und Mykologie Eberhard Karls Universität Auf der Morgenstelle 1 72076 Tübingen Germany;

    Physiologische Ökologie der Pflanzen Eberhard Karls Universität Auf der Morgenstelle 1 72076 Tübingen Germany;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ectomycorrhiza; Plant nitrogen nutrition; Ammonium; Amanita muscaria; Transport;

    机译:外生菌根;植物氮素营养;铵盐;毒蝇伞;运输;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号