Comparative Analysis of the Efficiency of Intron-Length Polymorphism of β-Tubulin Genes and Microsatellite Loci for Flax Varieties Genotyping

Rabokon A. N.; Pirko Ya. V.; Demkovych A. Ye.; Blume Ya. B.

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Comparative Analysis of the Efficiency of Intron-Length Polymorphism of β-Tubulin Genes and Microsatellite Loci for Flax Varieties Genotyping

机译：β-微管蛋白基因内含子长度多态性与微卫星基因座对亚麻品种基因分型效率的比较分析

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摘要

Efficacy of the beta-tubulin introns lenght polymorphism method (TBP) was used for Ukrainian bredeed flax cultivars genotyping. For this purpose, TBP data were compared with data produced using the two most effective species-specific SSR markers. Both methods were used to evaluate intra- and intercultivar flax polymorphism. For each cultivar, PIC data (Polymorphism Information Content) and the range of allele lenghts, as well as the number of allele phenotypes, were calculated using TBP and SSR markers. The dendrograms, built using Nei and Li's similarity coefficient, differ for SSR and TBP markers. Most flax cultivars of Ukrainian selection were genetically heterogeneous. The TBP method was highly efficient for differentiation of flax genotypes versus SSR analysis.

机译：β-微管蛋白内含子长度多态性方法（TBP）的功效用于乌克兰育种亚麻品种的基因分型。为此，将TBP数据与使用两种最有效的物种特异性SSR标记产生的数据进行了比较。两种方法都用于评估种内和种间亚麻多态性。对于每个品种，使用TBP和SSR标记计算PIC数据（多态信息内容）和等位基因长度的范围以及等位基因表型的数量。使用Nei和Li的相似系数构建的树状图在SSR和TBP标记上有所不同。乌克兰选择的大多数亚麻品种在遗传上是异质的。与SSR分析相比，TBP方法对区分亚麻基因型非常有效。

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来源
《Cytology and genetics》 |2018年第1期|1-10|共10页
作者
Rabokon A. N.; Pirko Ya. V.; Demkovych A. Ye.; Blume Ya. B.;
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作者单位

Natl Acad Sci Ukraine, Inst Food Biotechnol & Genom, Kiev, Ukraine;

Natl Acad Sci Ukraine, Inst Food Biotechnol & Genom, Kiev, Ukraine;

Natl Acad Sci Ukraine, Inst Food Biotechnol & Genom, Kiev, Ukraine;

Natl Acad Sci Ukraine, Inst Food Biotechnol & Genom, Kiev, Ukraine;

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正文语种 eng
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关键词
molecular-genetic markers; TBP (tubulin base polymorphism); SSR (simple sequence repeats); flax (Linum L.); DNA fingerprinting;

机译：分子遗传标记;TBP（微管蛋白碱基多态性）;SSR（简单序列重复）;亚麻（Linum L。）;DNA指纹图;

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1. Molecular Genetic Evaluation of Ukrainian Flax Cultivar Homogeneity Based on Intron Length Polymorphism of Actin Genes and Microsatellite Loci [J] . Postovoitova A. S., Yotka O. Yu., Pirko Ya. V., Cytology and genetics . 2018,第6期

机译：基于肌动蛋白基因内含子长度多态性和微卫星基因座的乌克兰亚麻品种同质性分子遗传评价
2. h-TBP: an approach based on intron-length polymorphism for the rapid isolation and characterization of the multiple members of the β-tubulin gene family in Camelina sativa (L.) Crantz [J] . Incoronata Galasso, Antonella Manca, Luca Braglia, Molecular Breeding . 2011,第4期

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3. h-TBP: an approach based on intron-length polymorphism for the rapid isolation and characterization of the multiple members of the beta -tubulin gene family in Camelina sativa (L.) Crantz. [J] . Galasso I., Manca A., Braglia L., Molecular Breeding . 2011,第4期

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Comparative Analysis of the Efficiency of Intron-Length Polymorphism of β-Tubulin Genes and Microsatellite Loci for Flax Varieties Genotyping

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