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首页> 外文期刊>Cytology and genetics >Induced autotetraploidy in Trachyspermum ammi (L.) Sprague (Apiaceae)
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Induced autotetraploidy in Trachyspermum ammi (L.) Sprague (Apiaceae)

机译：拟南芥（A.aceae）的诱导四倍体

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摘要

Polyploidization is an event that developed in plants during evolutionary path which confer them better survivorship than diploids towards the harsh environmental conditions. Regarding this approach polyploids acquire most of appealing facts in it which are the special concern of biologists. Present study is an attempt to induce autotetraploids in an important medicinal plant, Trachyspermum ammi (L.) Sprague through colchicine. The seedlings of Trachyspermum ammi (L.) Sprague were treated with 3 different concentrations of colchicine (0.2, 0.4 and 0.5%, w/v) for 3 different durations. The autotetraploidy in plants have been confirmed on the basis of cytological, morphological and palynological observations. Total six autotetraploid plants (4n = 36) were induced. On the basis of the number of induced autotetraploids, 36 hours duration of 0.2% concentration of colchicine was found to be more effective. The morphological parameters such as increased flower size, reproductive organs, stomata size, pollen length and diameter, plant height, leaf length and width, number of umbel/plant, number of umbellate/umbel and stomatal frequency etc. were considered for the detection of autotetraploids. Various chromosomal configurations have been observed during meiotic analysis. Pollen fertility was decreased in autotetraploids than diploids.
机译：多倍体化是植物在进化过程中发生的事件，在恶劣的环境条件下，它们比二倍体具有更好的生存能力。关于这种方法，多倍体获得了生物学家特别关注的大多数吸引人的事实。目前的研究是试图通过秋水仙碱在重要的药用植物Trachyspermum ammi（L.）Sprague中诱导同源四倍体的尝试。用3种不同浓度的秋水仙碱（0.2％，0.4％和0.5％，w / v）对Trachyspermum ammi（L.）Sprague的幼苗进行3种不同的持续时间处理。根据细胞学，形态学和孢粉学的观察，已经确认了植物中的四倍体。总共诱导了六种四倍体植物（4n = 36）。根据诱导的同源四倍体的数量，发现0.2％秋水仙碱的36小时持续时间更为有效。检测花的大小，生殖器官，气孔大小，花粉长度和直径，植物高度，叶长和宽度，伞形/植物的数量，伞形/伞形的数量和气孔频率等形态参数。同源四倍体。在减数分裂分析中已观察到各种染色体构型。同源四倍体的花粉生育力低于二倍体。

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