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首页> 外文期刊>Cytology and genetics >Multiplex PCR assay for detection of human somatotropin and interferon alpha2b genes in plant material
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Multiplex PCR assay for detection of human somatotropin and interferon alpha2b genes in plant material

机译：多重PCR法检测植物材料中人生长激素和干扰素α2b基因

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摘要

Using transgenic plants as factories for production of physiologically active human proteins arouses special concern because occasional escape of such transgenes into environment may cause health problems. Creation of plant varieties producing pharmaceutically valuable proteins should be accompanied by development of detection methods suitable for controlling the transgene behavior. Here we describe a multiplex PCR protocol for revealing of two human genes (encoding growth hormone and interferon alpha2b) that have been successfully introduced into plant genomes. The primer pair designed for detection of human growth hormone coding sequence amplifies fragments of different size from the full-length gene in the human genome and the intronless coding sequence usually used for plant transformation. Application of this primer pair may be recommended for ruling out false positive results due to sample contamination with human DNA. Such a control may be useful also in PCR analysis during establishing of transgenic plants carrying genes of human origin.
机译：将转基因植物用作生产具有生理活性的人类蛋白质的工厂引起了人们的特别关注，因为这种转基因偶尔逃逸到环境中可能会引起健康问题。产生可药用蛋白质的植物品种的创造应伴随着适于控制转基因行为的检测方法的发展。在这里，我们描述了一种多重PCR方案，用于揭示已成功引入植物基因组的两个人类基因（编码生长激素和干扰素α2b）。设计用于检测人类生长激素编码序列的引物对可扩增与人类基因组全长基因和通常用于植物转化的无内含子编码序列不同大小的片段。可能建议使用该引物对，以排除由于样品被人类DNA污染而导致的假阳性结果。在建立携带人源基因的转基因植物期间，这种对照在PCR分析中也可能有用。

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