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首页> 外文期刊>Cytology and genetics >Microsatellite analysis of chromosomes from the fifth homoeologous group in the introgressive Triticum aestivum/Amblyopyrum muticum wheat lines
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Microsatellite analysis of chromosomes from the fifth homoeologous group in the introgressive Triticum aestivum/Amblyopyrum muticum wheat lines

机译：渐进型小麦/小麦弱麦系第五系同源染色体染色体的微卫星分析

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摘要

Introgressive hybridization of common wheat has been and remains an efficient way for enriching its gene pool with resistance genes against environmental stress factors. In this aspect, T genome of the diploid species Amblyopyrum muticum (Boiss.) Eig. is believed to be prospective. The genome substitution amphidiploid Aurotica (AABBTT) with the A and B subgenomes from the common wheat cultivar Aurora and with the T subgenome from the indicated species is characterized by a high level of winter resistance. This trait is associated in wheat with the chromosomes of the fifth homoeologous group. Using a comparative microsatellite analysis of Aurora and Aurotica DNAs, we identified nine SSR loci specific for the wheat D genome, whose primers produced different amplicons from Aurotica DNA. The latter were used in screening F-5 plants (Aurora x Aurotica) for the search of the specimens that carried the 5T chromosome or its fragments in their genomes. Among 234 plants, we detected 24 plants with disomic 5D/5T substitutions, which were the progeny of five F-4 plants grown from two F-2 plants. A significant number of plants contained the recombinant 5D/5T chromosome. The recombination event could occur in any generation starting with F-2.
机译：普通小麦的渐渗杂交一直是并且仍然是利用抗环境胁迫因子的抗性基因丰富其基因库的有效途径。在这方面，二倍体物种Amblyopyrum muticum（Boiss。）Eig的T基因组。被认为是准的。来自普通小麦品种Aurora的A和B亚基因组以及所示物种的T亚基因组的基因组替代二倍体Aurotica（AABBTT）的特点是具有较高的冬季抗性。该性状在小麦中与第五同源群的染色体相关。通过对Aurora和Aurotica DNA进行比较微卫星分析，我们鉴定了小麦D基因组特异的9个SSR基因座，其引物从Aurotica DNA产生不同的扩增子。后者用于筛选F-5植物（Aurora x Aurotica），以搜索在基因组中携带5T染色体或其片段的标本。在234株植物中，我们检测到24种具有5D / 5T二体组置换的植物，它们是从两株F-2植物中生长出来的五株F-4植物的后代。大量植物含有重组5D / 5T染色体。从F-2开始的任何一代中都可能发生重组事件。

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