...
首页> 外文期刊>Cytology and genetics >Cytotoxic effects of metaphase-arresting methods in barley
【24h】

Cytotoxic effects of metaphase-arresting methods in barley

机译:大麦中期阻滞方法的细胞毒作用

获取原文
获取原文并翻译 | 示例
           

摘要

Metaphase-arresting agents amiprophos-methyl (APM), colchicine (COL) and cell cycle-synchronization (CCS) with APM and hydroxyurea (HU) were tested for growth, metaphase index and cytogenetic abnomalities in barley (Hordeum vulgare cv. Bornova-92). Seeds were germinated for 2 days and then seedlings were treated with 8 mu M (2.4 mg/L) APM for 2 h or 1.25 mM (0.5 g/L) COL or synchronized (CCS) with 1.25 mM (95 mg/L) hydroxyurea for 18 h and with 4 mu M (1.2 mg/L) APM for 2 h. APM and CCS caused metaphase indices 12.57 and 38.82% respectively. COL also arrested metaphase (14.10%) but also resulted in nuclear aberrations (11.15%). After removal of APM and CCS, cells were released to grow and divide. However, COL caused irreversible effects on cell division and growth and meanwhile was shown to be effective for micronucleus formation.
机译:测试了大麦(Hordeum vulgare cv。Bornova-92)大麦的生长,中期指标和细胞遗传学异常,检测了甲基丙烯酸甲酯(APM),秋水仙碱(COL)和与APM和羟基脲(HU)进行细胞周期同步(CCS)的中期抑制剂。 )。种子萌发2天,然后将幼苗用8μM(2.4 mg / L)APM处理2 h或1.25 mM(0.5 g / L)COL或同步(CCS)用1.25 mM(95 mg / L)羟基脲处理持续18小时,并用4μM(1.2 mg / L)APM持续2 h。 APM和CCS引起的中期指数分别为12.57和38.82%。 COL也逮捕了中期(14.10%),但也导致了核畸变(11.15%)。去除APM和CCS后,细胞被释放以生长和分裂。然而,COL对细胞分裂和生长产生不可逆的影响,同时被证明对微核形成有效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号