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首页> 外文期刊>Cytology and genetics >Production of a novel Robertsonian translocation from Thinopyrum bessarabicum into bread wheat
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Production of a novel Robertsonian translocation from Thinopyrum bessarabicum into bread wheat

机译：生产新型的罗伯逊氏易位，从甜菜丝藻到面包小麦

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摘要

Development of wheat-alien translocation lines will facilitate its practical utilization in wheat improvement. The objective of the present study was to produce compensating wheat-Thinopyrum bessarabicum whole arm Robertsonian translocations (RobTs) involving chromosomes 2B of wheat and 2E(b) of Th. bessarabicum through the mechanism of centric breakage-fusion. F-2 population from crosses between DS2E(b)(2B) substitution line and bread wheat "Roushan" (2n = 6x = 42, AABBDD) as female parent were made. Forty one F (2) lines (L1-L41) were screened for their chromosome composition. Three 2E(b) specific PCR-based Landmark Unique Gene (PLUG) markers were used for screening F (2) progeny derived from plants double-monosomic for chromosome 2B and 2E(b). Two RobTs (similar to 5%) were observed among F (2) plants. Homozygous translocation (T2E(b)S.2BL) with good plant vigor and full fertility were selected from F (3) families. The T2E(b)S.2BL stock has longer awn than that of its parents. It is cytogenetically stable, and may be useful in wheat improvement.
机译：小麦异种易位系的发展将促进其在小麦改良中的实际应用。本研究的目的是产生涉及小麦2B染色体和Th 2E（b）的补偿小麦-甜菜镰刀菌全臂罗伯逊易位（RobTs）。通过中心破损-融合机制来进行。从DS2E（b）（2B）替代系和作为母本的面包小麦“ Roushan”（2n = 6x = 42，AABBDD）之间的杂交获得F-2种群。筛选了41条F（2​​）品系（L1-L41）的染色体组成。三个基于2E（b）特异性PCR的地标唯一基因（PLUG）标记用于筛选F（2）后代，该后代来源于2B染色体和2E（b）染色体双单体植物。在F（2）种植物中观察到了两个RobT（约5％）。从F（3）家族中选择具有良好植物活力和完全育性的纯合易位（T2E（b）S.2BL）。 T2E（b）S.2BL股票的存货比其父母的存货更长。它具有细胞遗传学稳定性，可用于小麦改良。

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