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Induced Polyploidization in Brassica campestris L. (Brassicaceae)

机译:甘蓝型油菜(Brassicaceae)的诱导多倍体化

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摘要

Present experimental design has been made up to obtain crop with higher ploidy level via synthetic polyploidization. Since ploidy manipulation is generally associated with the obtainment of some increased enviable traits of the crop and also provides them greater adaptability to unfavorable or harsh circumstances as compared to its diploids counterparts. Thus, herein present research autotetraploids of Brassica campestris L. have been lucratively achieved by the application of colchicine. Two methods of treatment were utilized i.e. seed treatment and seedling treatment. No polyploidy could be obtained through seed treatment while seedling treatment responded well towards polyploidy. However, the status of autotetraploidy has been confirmed by cytomorphological investigations of treated plants as against its diploids counterparts. For the purpose, morphological parameters-sych as increased stomata size, pollen diameter, flower size, reproductive organs whereas reduction in plant height, leaf length, leaf breadth, stomata frequency, number of flowers/inflorescence etc. were appraised. Further, cytological observations were made that had clearly revealed the doubling of genome in the autotetraploids as compared to diploids. Meanwhile, pollen fertility and size of pollen grains were evaluated as well.
机译:目前的实验设计已经准备好,通过合成多倍体化获得具有更高倍性水平的农作物。由于倍性操纵通常与获得一些增加的令人羡慕的农作物性状相关,并且与二倍体对应物相比,还为它们提供了更好的适应不利或恶劣环境的能力。因此,在本文中,通过使用秋水仙碱有利地获得了甘蓝型油菜的研究四倍体。使用了两种处理方法,即种子处理和幼苗处理。通过种子处理无法获得多倍性,而幼苗处理对多倍性反应良好。但是,通过四倍体处理植物的细胞形态学研究已经证实了其四倍体的状况。为此,评估了形态学参数,如气孔大小,花粉直径,花大小,生殖器官的增加,而植物高度,叶长,叶宽,气孔频率,花/花序数等的减少。此外,进行的细胞学观察清楚地揭示了与二倍体相比,同源四倍体中基因组的倍增。同时,还评估了花粉的育性和花粉粒的大小。

著录项

  • 来源
    《Cytology and genetics》 |2014年第2期|103-110|共8页
  • 作者

    G. Kumar; K. Dwivedi;

  • 作者单位

    Plant Genetics Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002 India;

    Plant Genetics Laboratory, Department of Botany, University of Allahabad, Allahabad, 211002 India;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    autotetraploid; Brassica campestris L.; colchicine; diploid;

    机译:同源四倍体甘蓝型油菜;秋水仙碱;二倍体;

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