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首页> 外文期刊>Cytology and genetics >Agrobacterium-Mediated Transformation of Sunflower (Helianthus annuus L.) in vitro and in Planta Using Lba4404 Strain Harboring Binary Vector pBi2E with dsRNA-Suppressor of Proline Dehydrogenase Gene
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Agrobacterium-Mediated Transformation of Sunflower (Helianthus annuus L.) in vitro and in Planta Using Lba4404 Strain Harboring Binary Vector pBi2E with dsRNA-Suppressor of Proline Dehydrogenase Gene

机译：农杆菌介导的Lba4404带有二元载体pBi2E的脯氨酸脱氢酶基因的dsRNA抑制剂体外和在植物中转化向日葵（Helianthus annuus L.）

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摘要

To estimate the efficiency of proline dehydrogenase gene suppression aimed at increasing sunflower (Helianthus annuus L.) tolerance level to water deficiency and salinity, we employed the LBA4404 strain harboring pBi2E with double-stranded RNA-suppressor, produced based on the ProDH1 gene of Arabidop-sis. Techniques for Agrobacterium-mediated transformation in vitro and in planta during fertilization of sunflower have been proposed. The genotype-dependent integration of T-DNA in the sunflower genome was demonstrated. PCR-analysis showed that ProDH1 is present in the genomes of inbred lines transformed in planta, as well as in T1- and T2-generations. Significantly, increased levels of accumulation of free L-proline during early stages of in vitro cultivation under normal conditions were demonstrated in transgenic regenerants. The application of lethal doses of stressors (0.4 M mannitol and 2.0% sea salts) caused increase of L-proline level in transgenic regenerants and its decline during the recovery period. This data indicates the effectiveness of suppression of the sunflower ProDH1 gene for increased osmotolerance.
机译：为了评估脯氨酸脱氢酶基因抑制的效率，旨在提高向日葵（Helianthus annuus L.）对缺水和盐度的耐受水平，我们使用了带有pBi2E的LBA4404菌株和双链RNA抑制剂，该菌株基于拟南芥的ProDH1基因生产-sis已经提出了在向日葵施肥过程中农杆菌介导的体外和植物转化的技术。证明了向日葵基因组中T-DNA的基因型依赖性整合。 PCR分析表明ProDH1存在于植物中转化的近交系的基因组中，以及T1和T2世代中。显着地，在转基因再生体中，在正常条件下体外培养的早期阶段，游离L-脯氨酸的积累水平增加。致死剂量的应激源（0.4 M甘露醇和2.0％海盐）的使用导致转基因再生体中L-脯氨酸水平升高，并在恢复期间下降。该数据表明抑制向日葵ProDH1基因对于增加渗透压的有效性。

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  • 来源
    《Cytology and genetics》 |2014年第4期|218-226|共9页
  • 作者

    O. M. Tishchenko; A. G. Komisarenko; S. I. Mykhalska; L. E. Sergeeva; N. I. Adamenko; B. V. Morgun; A. V. Kochetov;

  • 作者单位

    Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine,ul. Vasylkivska 31/17, Kyiv, 03022 Ukraine;

    Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine,ul. Vasylkivska 31/17, Kyiv, 03022 Ukraine;

    Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine,ul. Vasylkivska 31/17, Kyiv, 03022 Ukraine;

    Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine,ul. Vasylkivska 31/17, Kyiv, 03022 Ukraine;

    Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine,ul. Vasylkivska 31/17, Kyiv, 03022 Ukraine;

    Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine,ul. Vasylkivska 31/17, Kyiv, 03022 Ukraine,Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine,ul. Akademika Zabolotnogo 148, Kyiv, 03680 Ukraine;

    Institute of Cytology and Genetics, Russian Academy of Sciences,ul. Akademika Lavrent'eva 10, Novosibirsk, 630090 Russia;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    sunflower (Helianthus annuus L.); Agrobacterium-mediated transformation in vitro and in planta; dsRNA-suppressor of proline dehydrogenase;

    机译：向日葵（Helianthus annuus L.）;农杆菌介导的体外和植物转化;脯氨酸脱氢酶的dsRNA抑制剂;

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