INFLUENCE OF EXTRACT OF CULTURED CELLS OF Ungernia victoris AND CATIONS OF CERTAIN METALS ON THE EFFICIENCY OF TRANSFORMATION OF Escherichia coli CELLS BY PLASMID DNA

A. Yu. Miryuta; T. P. Pererva; L. P. Mozhilevskaya; V. A. Kunakh

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INFLUENCE OF EXTRACT OF CULTURED CELLS OF Ungernia victoris AND CATIONS OF CERTAIN METALS ON THE EFFICIENCY OF TRANSFORMATION OF Escherichia coli CELLS BY PLASMID DNA

机译：vic虫培养细胞提取物和某些金属阳离子对质粒DNA转化大肠杆菌细胞效率的影响

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The influence of an extract of biomass of U. victoris cells cultured in vitro on the relation between the yield of transformants, ceD survival, and changes in the conformation of plasmid DNA is studied in the transformation system of E. coli treated with CaCl_2 using the DNA of the plasmid pBR322. The data obtained are compared to the influence of certain salts of mono- and bivalent metals on these parameters. It is shown that there exist different mechanisms responsible for variations in the survival rate of a cell population and yield of transformants depending on whether salts or the plant extract is used.

机译：利用CaCl_2处理的大肠杆菌转化体系，研究了体外培养的victoris细胞生物量提取物对转化子产量，ceD存活率和质粒DNA构象变化之间关系的影响。质粒pBR322的DNA。将获得的数据与某些一价和二价金属盐对这些参数的影响进行比较。结果表明，取决于使用盐还是植物提取物，存在不同的机制来负责细胞群体的存活率和转化体产量的变化。

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《Cytology and genetics》 |2005年第6期|p.22-28|共7页
作者
A. Yu. Miryuta; T. P. Pererva; L. P. Mozhilevskaya; V. A. Kunakh;
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Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Ul. Akademik Zabolotnyy 150, Kiev 03143 (Ukraine);

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正文语种 eng
中图分类普通生物学;
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1. Influence of plasma-generated reactive species on the plasmid DNA structure and plasmid-mediated transformation of Escherichia coli cells [J] . Lee Geonjoon, Choi Minah, Kim Daewook, Journal of Applied Physics . 2017,第10期

机译：血浆生成的反应性物种对大肠杆菌细胞质粒DNA结构和质粒介导转化的影响
2. Influence of plasma-generated reactive species on the plasmid DNA structure and plasmid-mediated transformation of Escherichia coli cells [J] . Lee Geon Joon, Choi Min Ah, Kim Daewook, Journal of Geophysical Research. Biogeosciences . 2017,第10期

机译：等离子体产生的反应性物种对大肠杆菌细胞质粒DNA结构和质粒介导的影响
3. Plasmid DNA Transformation in Escherichia Coli: Effect of Heat Shock Temperature, Duration, and Cold Incubation of CaCl_2 Treated Cells [J] . Mahipal Singh, Arpita Yadav, Xiaoling Ma, International Journal of Biotechnology and Biochemistry . 2010,第4期

机译：大肠杆菌中的质粒DNA转化：热休克温度，持续时间和CaCl_2处理细胞的冷培养的影响
4. Purification of plasmid DNA from Escherichia coli lysate using superparamagnetic nanoparticles modified with amino functional groups [C] . X.L. Qiu, Y.P. Guan, K.K. Wang, International Conference on Advanced Materials, Structures and Mechanical Engineering . 2016

机译：使用氨基官能团改性的超顺磁纳米粒子从大肠杆菌裂解物纯化质粒DNA
5. Mass spectrometric analysis of the heme cofactor of cytochrome P450(BM-3) in Escherichia coli HU-227 cells, and, Increasing P450 expression in HU-227 cells using transformation experiments. [D] . Lawson, Alin A. 2006

机译：使用转化实验对大肠杆菌HU-227细胞中细胞色素P450（BM-3）血红素辅因子进行质谱分析，并提高HU-227细胞中P450表达。
6. Replication of a low-copy-number plasmid by a plasmid DNA-membrane complex extracted from minicells of Escherichia coli. [O] . W Firshein, P Strumph, P Benjamin, 1982

机译：从大肠杆菌小细胞提取的质粒DNA-膜复合物复制低拷贝数的质粒。
7. Recombination efficiency is a quadratic function of the length of homology during plasmid transformation of Bacillus subtilis protoplasts and Escherichia coli competent cells. [O] . B. Michel, S.D. Ehrlich 1984

机译：重组效率是枯草芽孢杆菌原生质体和大肠杆菌富集细胞的质粒转化期间同源性长度的二次函数。

INFLUENCE OF EXTRACT OF CULTURED CELLS OF Ungernia victoris AND CATIONS OF CERTAIN METALS ON THE EFFICIENCY OF TRANSFORMATION OF Escherichia coli CELLS BY PLASMID DNA

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