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首页> 外文期刊>Cytology and genetics >Comparison of Alleles at Gli-2 Loci of Common Wheat by Means of Two-Dimensional Electrophoresis of Gliadin
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Comparison of Alleles at Gli-2 Loci of Common Wheat by Means of Two-Dimensional Electrophoresis of Gliadin

机译：麦醇溶蛋白二维电泳比较普通小麦Gli-2位点的等位基因

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摘要

Electrophoretic mobility (EM) and molecular weight (MW) of some allelic variants of alpha- and beta-gliadins contrlled by Gli-2 loci were compared by means of two-dimensional (APAGE x SDS) electrophoresis. Comparison of alpha-gliadins of the alleles Gli-A2b and Gli-A2p, of beta-gliadins of the Gli-B2b and Gli-B2c, and of beta-gliadins of the Gli-D2b, Gli-D2c, Gli-D2j, and Gli-D2r indicated that a gliadin with lower EM had, as a rule, bigger MW which is known to depend on the length of the polyglutamine domain of gliadin of alpha-type. However, allelic variants of the alpha-gliadin encoded by Gli-D2b and Gli-D2e differ in EM but not in apparent MW. It might be caused by a substitution of some charged/uncharged aminoacids in the polypeptide of gliadin. Allele Gli-B2o which is very frequent in up-to-date common wheat germplasm originated probably by means of unequal crossingover. Some alleles at Gli-A2 is found to control completely different blocks of gliadins and therefore might come to common wheat from different genotypes of the polymorphic diploid donor of the A genome. The results indicate that the reason of the known more vast polymorphism of gliadins controlled by Gli-2 loci as compared with Gli-1 loci is the considerable difference of the structure, first, of Gli-1 and Gli-2 loci (Gli-2 loci have more expressed genes per locus) and, second, of genes encoding gliadins of alpha- and gamma-types (alpha-gliadins are shown to contain a long polyglutamine sequences highly variable in their length).
机译：通过二维（APAGE x SDS）电泳比较了Gli-2基因座控制的一些α-和β-麦醇溶蛋白等位基因变体的电泳迁移率（EM）和分子量（MW）。等位基因Gli-A2b和Gli-A2p的α-麦醇溶蛋白，Gli-B2b和Gli-B2c的β-麦醇溶蛋白以及Gli-D2b，Gli-D2c，Gli-D2j和β-麦醇溶蛋白的比较Gli-D2r表明，具有较低EM的麦醇溶蛋白通常具有较大的MW，已知其取决于α型麦醇溶蛋白的聚谷氨酰胺结构域的长度。然而，由Gli-D2b和Gli-D2e编码的α-麦醇溶蛋白的等位基因变体在EM上不同，但在表观MW上没有差异。这可能是由于麦醇溶蛋白多肽中某些带电/不带电氨基酸的取代引起的。等位基因Gli-B2o在最新的普通小麦种质中非常常见，可能是由于不平等的杂交而产生的。发现Gli-A2的某些等位基因控制完全不同的麦醇溶蛋白块，因此可能来自A基因组多态二倍体供体的不同基因型的普通小麦。结果表明，已知与Gli-1基因座相比，受Gli-2基因座控制的麦醇溶蛋白具有更大的多态性的原因是结构上的巨大差异，首先是Gli-1和Gli-2基因座（Gli-2基因座每个基因座具有更多表达的基因），其次是编码α-和γ-型麦醇溶蛋白的基因（已显示α-麦醇溶蛋白含有长的聚谷氨酰胺序列，其长度可变。）

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