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首页> 外文期刊>Cytology and genetics >Accumulation of Indolyl-3-Acetic and Abscisic Acids by 'Hairy' Roots of Artemisia vulgaris
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Accumulation of Indolyl-3-Acetic and Abscisic Acids by 'Hairy' Roots of Artemisia vulgaris

机译：寻常蒿的多毛根积累吲哚基-3-乙酸和脱落酸

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High-performance liquid chromatography-mass spectrometry has been used to study the character of the accumulation and balance of endogenous indolyl-3-acetic (IAA) and abscisic (ABA) acids in the hairy roots of Artemisia vulgaris obtained by transformation with Agrobacterium rhizogenes agropine strain A4. The free IAA content was significantly higher than the level of the conjugated form of the hormone. The highest amount of the active IAA form (554.4 +/- 27.7 ng/g fresh weight) was detected in line no. 2. The free form of ABA predominated in the roots of control samples, where its level was significantly higher than in the transformed lines. The hairy roots accumulated a conjugated form of ABA, and the highest content of this compound amounted to 459.6 +/- 23.0 ng/g of fresh weight (in line no. 4). The architecture of the transformed roots was marked by significant branching and lateral root formation; active accumulation of biomass was detected. The results obtained demonstrate changes in the endogenous phytohormone balance in the hairy A. vulgaris roots, namely, a trend to predomination of the free IAA form and a significant decrease of the ABA content.
机译：高效液相色谱-质谱法已用于研究用发根农杆菌农杆菌转化获得的寻常蒿的毛状根中内源吲哚基-3-乙酸（IAA）和脱落酸（ABA）的积累和平衡特征菌株A4。游离IAA含量显着高于激素结合形式的水平。在第1行中检测到最高量的活性IAA形式（554.4 +/- 27.7 ng / g新鲜重量）。 2.游离形式的ABA在对照样品的根中占优势，其水平显着高于转化株系。毛状根累积了ABA的结合形式，该化合物的最高含量为鲜重的459.6 +/- 23.0 ng / g（第4行）。转化根的结构以明显的分支和侧根形成为特征。检测到生物质的活跃积累。所获得的结果表明，毛状芦荟根中内源性植物激素平衡发生变化，即游离IAA形式占优势的趋势和ABA含量显着下降。

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