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首页> 外文期刊>Cytology and genetics >Placenta Growth Factor Influences miR-483-5p, miR-483-3p, miR-4669 and miR-16-5p Expression in MKN-45-Derived Spheroid Body-Forming Cells
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Placenta Growth Factor Influences miR-483-5p, miR-483-3p, miR-4669 and miR-16-5p Expression in MKN-45-Derived Spheroid Body-Forming Cells

机译：胎盘生长因子影响MKN-45衍生球体形成细胞中miR-483-5p，miR-483-3p，miR-4669和miR-16-5p的表达

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摘要

Placenta growth factor (PlGF) is a crucial player of the human gastric cancer development. PlGF signalling pathway affects the expression of genes involving in angiogenesis and metastasis. Studies have hinted association between abnormal intracellular signal transduction and miRNAs expression profile in cancer initiation and progression. Changes in the expression of miR-483-5p, miR-483-3p, miR-16-5p and miR-4669 are reported in the spheroid body (SB)-forming cells derived from gastric cancer cell line MKN-45. Given the importance of PlGF and also the expression change of the above mentioned miRNAs in gastric cancer, this study was designed to investigate the effect of siRNA-mediated knockdown of Plgf on the expression of these miRNAs in the MKN-45 derived SB-forming cells. In addition, bioinformatics analysis was performed on the miRNAs to predict their potential targets that associated with survival, apoptosis and angiogenesis processes. Results showed that with except miR-483-3p, which was down-regulated, another 3 miRNAs were significantly up-regulated in the Plgf-knockdown samples. Furthermore, the in silico analysis revealed that these miRNAs influence the expression of a set of genes, which are involved in various signal transduction pathways. Moreover, it showed that they affect cellular processes, including proliferation, apoptosis and angiogenesis. In conclusion, the current study reveals that down-regulation of Plgf influences the miRNAs expression in MKN-45 derived SB-forming cells. Moreover, our findings indicate that miR-483-5p, miR-483-3p and miR-16-5p can induce cancer initiation and progression through targeting genes involved in the cell cycle, apoptosis and angiogenesis processes.
机译：胎盘生长因子（PlGF）是人类胃癌发展的关键因素。 PlGF信号传导途径影响涉及血管生成和转移的基因的表达。研究表明，异常细胞内信号转导与miRNAs表达谱在癌症的发生和发展之间存在关联。据报道，在源自胃癌细胞系MKN-45的球形体（SB）形成细胞中，miR-483-5p，miR-483-3p，miR-16-5p和miR-4669的表达发生了变化。考虑到PlGF的重要性以及上述miRNA在胃癌中的表达变化，本研究旨在研究siRNA介导的Plgf敲低对MKN-45衍生的SB形成细胞中这些miRNA表达的影响。 。此外，对miRNA进行了生物信息学分析，以预测其与生存，凋亡和血管生成过程相关的潜在靶标。结果显示，除了miR-483-3p被下调外，Plgf敲除样品中还有3个miRNA被上调。此外，计算机分析表明，这些miRNA影响一组基因的表达，这些基因参与各种信号转导途径。而且，它表明它们影响细胞过程，包括增殖，凋亡和血管生成。总之，当前的研究揭示了Plgf的下调会影响MKN-45衍生的SB形成细胞中miRNA的表达。此外，我们的发现表明，miR-483-5p，miR-483-3p和miR-16-5p可以通过靶向参与细胞周期，凋亡和血管生成过程的基因来诱导癌症的发生和发展。

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