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首页> 外文期刊>Cytology and genetics >Development of Novel Low-Copy Nuclear Gene Primers by Transcriptomes in an Endemic Genus Orinus Hitchc. (Poaceae) from the Qinghai-Tibet Plateau
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Development of Novel Low-Copy Nuclear Gene Primers by Transcriptomes in an Endemic Genus Orinus Hitchc. (Poaceae) from the Qinghai-Tibet Plateau

机译：特有属Orinus Hitchc中转录组的新型低拷贝核基因引物的开发。 （禾本科）来自青藏高原

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摘要

In the present study, a total of eleven low-copy nuclear gene markers were developed using transcriptomes for the plant genus Orinus Hitchc. (Poaceae), which comprises three species of grasses widely distributed in the Qinghai-Tibet Plateau. These developed markers were successfully amplified and sequenced for 90 individuals from nine populations of three Orinus species. The DNA sequences for the eleven loci ranged from 606 to 1152 aligned based pairs. Compared with four candidate DNA barcoding markers in our previous studies, the newly developed markers had a relatively higher number of parsimony informative sites than three cpDNA spacers (7-20 vs. 1-8), but slightly lower than those of the internal transcribed spacers (ITS). Hence, these novel low-copy nuclear gene primer pairs were validly useful for the study of phylogeography, phylogenetic analysis and population genetics. Moreover, they could be used in phylogeny reconstruction and speciation for a broad range of Orinus and even the entire subtribe Tridentinae.
机译：在本研究中，利用转录组为植物属Orinus Hitchc开发了总共11个低拷贝核基因标记。 （禾本科），由在青藏高原广泛分布的三种草组成。这些发达的标记已成功扩增，并从三个Orinus物种的9个种群的90个个体中测序。 11个基因座的DNA序列范围从606到1152基于比对的对。与我们先前的研究中的四个候选DNA条形码标记相比，新开发的标记具有比三个cpDNA间隔子相对较高的简约信息位点（7-20对1-8个），但比内部转录的间隔子要低一些（它的）。因此，这些新颖的低拷贝核基因引物对可有效用于系统地理学，系统发育分析和种群遗传学研究。此外，它们可用于大范围的Orinus甚至整个Tridentinae部落的系统发育重建和物种形成。

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  • 来源
    《Cytology and genetics》 |2019年第3期|261-265|共5页
  • 作者

    Liu Yuping; Su Xu; Lv Ting; Chen Kelong;

  • 作者单位

    Qinghai Normal Univ, Sch Life Sci, Key Lab Med Plant & Anim Resources Qinghai Tibet, Xining 810008, Peoples R China|Qinghai Normal Univ, Sch Life Sci, Key Lab Phys Geog & Environm Proc Qinghai Prov, Xining 810008, Peoples R China;

    Qinghai Normal Univ, Sch Life Sci, Key Lab Med Plant & Anim Resources Qinghai Tibet, Xining 810008, Peoples R China|Qinghai Normal Univ, Sch Life Sci, Key Lab, Educ Minist Environm & Resources Qinghai Tibet Pl, Xining 810008, Peoples R China;

    Qinghai Normal Univ, Sch Life Sci, Key Lab Med Plant & Anim Resources Qinghai Tibet, Xining 810008, Peoples R China|Qinghai Normal Univ, Sch Life Sci, Key Lab Phys Geog & Environm Proc Qinghai Prov, Xining 810008, Peoples R China;

    Qinghai Normal Univ, Sch Life Sci, Key Lab, Educ Minist Environm & Resources Qinghai Tibet Pl, Xining 810008, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Poaceae; Orinus; transcriptome; low-copy nuclear gene; primer; next-generation sequencing;

    机译：Poaceae;orinus;转录组;低拷贝核基因;底漆;下一代测序;

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