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Genetic Modification of Saccharum officinarum for Herbicide Tolerance

机译：除草剂耐受性糖素素的遗传修饰

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Sugarcane is one of the most important worldwide cultivated agro-industrial crops that belong to the family Poaeceae and genus Saccharum. Prescribed study was conducted in Seed Biotechnology Lab, Center of Excellence in Molecular Biology, University of the Punjab, Lahore-Pakistan. The purpose of this study was to alter the genome of sugarcane line CPF246 with herbicide resistance gene GTGene driven by the sugar cane ubiquitin promoter and confirmation through PCR and Dot blot as well as protein expression analysis through ELISA. Results have shown the successful transformation and expression of transgenes against glyphosate resistance. This could prove a mile stone towards putting research efforts in the improvement of Saccharum officinarum for certain traits. Outcomes of this research will be beneficial in minimizing the management cost which people do bear for weed management practices. Success of this study opens new horizons to further improve other characteristics of sugarcane using genetic modification as was done in current study.
机译：甘蔗是全球最重要的培养农业作物之一，属于Poaeceae和Genus Saccharum。规定的研究是在种子生物技术实验室中进行，旁遮普巴基斯坦旁遮普大学的分子生物学卓越中心。本研究的目的是改变甘蔗线CPF246的基因组，其与甘蔗泛素启动子驱动的除草剂抗性基因Gtgene并通过ELISA通过PCR和点印迹确认以及蛋白质表达分析。结果表明，转基因对草甘膦抗性的成功转化和表达。这可以证明一英里的石头在改善Saccharum Officinarum以实现某些特征的研究。这项研究的结果将有利于最小化人们为杂草管理实践承担的管理成本。本研究的成功开启了新的视野，以进一步改善甘蔗的其他特征，使用当前研究中的遗传修饰。

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