...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytology and genetics >Association of the ACE (rs1800764) Polymorphism with Risk of Diabetic Kidney Disease in Saudi Arabian Population: A Pilot Study using the PCR-RFLP Method
【24h】

Association of the ACE (rs1800764) Polymorphism with Risk of Diabetic Kidney Disease in Saudi Arabian Population: A Pilot Study using the PCR-RFLP Method

机译：ACE（RS1800764）多态性与沙特阿拉伯人群糖尿病肾病风险的关联：使用PCR-RFLP方法的试验研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Diabetic kidney disease (DKD) also known as diabetic nephropathy is the leading cause of end-stage renal disease, with multiple genetic and environmental factors involving in its etiology. Angiotensin-converting enzyme (ACE) gene is considered to have an important role in the development and progression of DKD. In this case-control study, we investigated the role of ACE T3892C (rs1800764) polymorphism in the development of DKD in Saudi Arabian population. We recruited 150 type 2 diabetic cases with DKD and 150 type 2 diabetic controls without DKD. The differences in age, sex, systolic blood pressure (SBP), diastolic blood pressure (DBP), duration of diabetes, fasting blood glucose, urinary albumin, albumin/creatinine ratio, serum urea and serum-creatinine between the two groups were analyzed. The genotyping of ACE T3892C polymorphism was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Genotype and allele frequencies were calculated by direct counting. Deviations from Hardy-Weinberg equilibrium (HWE) were tested using the Chi-square (chi 2) test in both of studied groups. Odds ratio (OR) with 95% CI were used to evaluate the relationship of ACE T3892C polymorphism with DKD susceptibility. Statistical analysis was performed using SPSS (version 21.0) software and Medcalc software (version 16.4.3). The frequency distribution of ACE T3892C polymorphism was found to be different between case and control groups significantly indicating ACE gene could play an important role in the pathogenesis of DKD in Saudi Arabian population.
机译：糖尿病肾病（DKD）也称为糖尿病肾病是终末期肾病的主要原因，具有涉及其病因的多种遗传和环境因素。血管紧张素转化酶（ACE）基因被认为在DKD的开发和进展中具有重要作用。在这种情况下，我们调查了ACE T3892C（RS1800764）多态性在沙特阿拉伯人口发展中的作用。我们招募了150型2型糖尿病病例，DKD和150型没有DKD的2型糖尿病患者。分析了年龄，性别，收缩压（SBP），舒张压（DBP），糖尿病持续时间，禁食血糖，尿白蛋白，白蛋白/肌酐比，血清尿素和血清 - 在两组之间两组之间的差异。使用聚合酶链反应限制片段长度多态性（PCR-RFLP）方法进行ACE T3892C多态性的基因分型。通过直接计数计算基因型和等位基因频率。使用研究组中的Chi-Square（CHI 2）试验测试了来自HARDY-WEINBERG平衡（HWE）的偏差。使用95％CI的差距比（或）用于评估ACE T3892C多态性与DKD易感性的关系。使用SPSS（版本21.0）软件和MEDCALC软件（16.4.3版）进行统计分析。发现ACE T3892C多态性的频率分布在显着表明ACE基因可能在沙特阿拉伯人群中发病机制中发挥着重要作用的案例和对照组之间存在差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号