Calcium-Dependent Changes in Cellular Redox Homeostasis and Heat Resistance of Wheat Plantlets under Influence of Hemin (Carbon Monoxide Donor)

Shkliarevskyi M. A.; Karpets Yu. V.; Kolupaev Yu. E.; Lugovaya A. A.; Dmitriev A. P.

摘要

Carbon monoxide (CO) is considered to be an important molecule-gasotransmitter involved in the regulation of the functional activity of plants, including in the processes of adaptation to stress factors. However, the associations of CO with other participants of signaling in the plant cells remain poorly studied. Using an inhibitory method, the role of different calcium pools in realization of the influence of hemin (carbon monoxide donor) on generation and neutralization of reactive oxygen species (ROS) in the root cells of wheat (Triticum aestivum L.) plantlets and their resistance to damaging heating (45 degrees C, 10 min) was studied. The treatment of plantlets with 5 mu M hemin caused a transient increase in the activity of extracellular peroxidase in roots and an increase in the generation of ROS with the maximum in 1.5-2 h after the beginning of treatment. The chelator of extracellular calcium EGTA and the inhibitor of inositol-1,4,5-phosphate formation neomycin, which reduces calcium influx into the cytosol from intracellular compartments, almost completely eliminated an increase in the activity of extracellular peroxidase caused by exogenous CO. At the same time, EGTA (completely) and neomycin (partially) leveled an increase in the content of hydrogen peroxide in the roots of plantlets occurring under the influence of CO donor. The treatment of plantlets with hemin also induced an increase in the activity of superoxide dismutase, catalase, and intracellular peroxidase in roots. Both calcium antagonists eliminated these effects. In the presence of EGTA and neomycin, there was also no positive effect of the treatment of hemin on the state of biomembranes and survival of plantlets after the damaging heating. It was concluded that both extracellular calcium and that deposited in intracellular compartments is involved in CO donor-induced increase in the formation of ROS, induction of the antioxidant system, and development of heat resistance of wheat plantlets.

机译：一氧化碳（CO）被认为是涉及植物功能活性的重要分子 - 气体转手，包括适应应力因子的过程。然而，CO与其他参与者在植物细胞中的其他参与者的关联研究仍然很差。使用抑制方法，在小麦（Triticum aestivum L.）植物（Triticum aestivum L.）植物的根细胞中对血红素（一氧化碳供体）对血红素（一氧化碳供体）对反应性氧物种（ROS）产生和中和的作用及其抵抗力的作用及其抗性研究了损坏的加热（45摄氏度，10分钟）。用5μmhemin的植物治疗引起了根系细胞外过氧化物酶活性的瞬时增加，并且在治疗开始后的1.5-2小时内的产生增加。细胞外钙EGTA的螯合剂和肌醇-1,4,5-磷酸盐形成新霉素的抑制剂，从而将钙流入细胞内隔室中的钙流入胞嘧啶，几乎完全消除了外源CO引起的细胞外过氧化物酶活性的增加。在同时，EGTA（完全）和新霉素（部分）在福服供体的影响下发生的植物根部的根部中的过氧化氢含量的增加。用血红素的植物治疗还诱导了超氧化物歧化酶，过氧化氢酶和根茎过氧化物酶的活性增加。钙拮抗剂都消除了这些效果。在EGTA和Neomycin的存在下，在损伤加热后，血红素治疗血红素的治疗和植物植物的存活率也没有积极效应。结论是，细胞外钙和沉积在细胞内隔室中的含量诱导的CO供体诱导的ROS诱导的抗氧化系统的诱导和小麦植物耐热性的发展。

Calcium-Dependent Changes in Cellular Redox Homeostasis and Heat Resistance of Wheat Plantlets under Influence of Hemin (Carbon Monoxide Donor)

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