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首页> 外文期刊>Cytology and genetics >Genetic Variability in Winter Rye (Secale cereale L.) Accessions at Early Stage of Self-Pollination Manifested through Fertility, Plant Height and Secalins
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Genetic Variability in Winter Rye (Secale cereale L.) Accessions at Early Stage of Self-Pollination Manifested through Fertility, Plant Height and Secalins

机译：冬季Rye遗传变异（Secale Cereale L.）在自我授粉的早期阶段的避孕，通过生育，植物高度和Secalins表现出

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Selection of winter self-pollinated plants with short to medium stem height was initiated in 15 cultivated rye populations. About 8.8% seed set per selfed spike was registered in the first two years. In inbred S-2 families, self-fertility varied between 0-73 seeds per isolated ear and plant height ranged from 76 to 115 cm. Response to selection and genetic advance in percent of the mean characterized the S-3 families for one cycle of selection. SDS-PAGE gel patterns showed two major protein bands for the rye HMW secalins-one slowly moving x-subunit expressed as 2r, 5.2*r and 5.3r, and the second quickly moving y-subunit, expressed as 6r, 6.5r, 7r and 9r. Regarding alleles in Glu-R1 and Gli-R2 loci, nine progenies appeared to show genetic homogeneity in proteins, supporting by low coefficients of variation for plant height. The HMW compositions 2r (alone band) and 5.3r + 7r, were defined as new secalin subunits. 75 K gamma-secalins, encoded at Gli-R2, were composed by alleles a, b, c, for subunits d1, d2 and t1, respectively. The results showed that among all, four selfed rye progenies were considered as homogeneous genotypes and could be used as inbred lines in further genetic and breeding experiments.
机译：在15种栽培的黑麦种群中开始选择短至中茎高的冬季自授粉植物。每次自行钉的约8.8％的种子套装在前两年注册。在近交的S-2家庭中，自生育能力在0-73种耳朵之间变化，植物高度范围为76至115厘米。响应选择和遗传提前的平均值的百分比表征了一个选择的一个循环的S-3系列。 SDS-PAGE凝胶模式显示了黑麦HMW Secalins的两个主要蛋白质带 - 一个缓慢移动的X-亚基表示为2R，5.2 * R和5.3R，第二个快速移动的Y-亚基表示为6R，6.5R，7R和9r。关于Glu-R1和Gli-R2基因座的等位基因，九个后代似乎在蛋白质中显示出遗传均匀性，通过植物高度的低系数支撑。 HMW组合物2R（单独带）和5.3r + 7r，定义为新的Secalin亚基。在GLI-R2上编码的75kγ-kema-secalin由等位基因A，B，C，分别用于亚基D1，D2和T1。结果表明，其中四个自带黑麦产后被认为是均相基因型，可用作进一步遗传和育种实验中的自交系。

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