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Influence of anoxic to aerobic volume ratio on sludge settleability and bacterial community structure in a denitrifying-nitrifying activated sludge system

机译:厌氧/好氧体积比对反硝化-硝化活性污泥系统污泥沉降性和细菌群落结构的影响

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摘要

Biological treatment processes under nitrifying and denitrifying conditions are complex systems, in which different kinds of microbes dynamically cultivate and interact. The sludge bulking in these systems can be controlled by understanding the relationship between reactor performance and microbial diversity. In this study, sludge settleability and bacterial community structure were investigated in a modified Ludzack-Ettinger system as anoxic to aerobic volume ratio increased from 1:3 to 1:2 and finally to 2:1. Results indicated that settleability significantly improved as the anoxic to aerobic volume ratio increased (p<0.01), accompanied by the increase in nitrogen removal efficiency and the change in filamentous bacteria. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis revealed that the diversity and richness of bacterial species increased with a small volume ratio but decreased as the volume ratio increased, because of the low energy obtained from anoxic respiration. Proteobacteria were the predominant group, followed by Bacteroidetes. The influence of the anoxic condition on bacterial community structure was evaluated through correspondence analysis. The changes in bacterial community structure concurred with the reactor performance in terms of nitrogen removal efficiency and settleability. Samples with different settleability values were inclined to cluster into different groups.
机译:硝化和反硝化条件下的生物处理过程是复杂的系统,其中不同种类的微生物动态地培养和相互作用。通过了解反应堆性能和微生物多样性之间的关系,可以控制这些系统中的污泥膨胀。在这项研究中,在改良的Ludzack-Ettinger系统中研究了污泥的沉降性和细菌群落结构,因为缺氧与好氧的体积比从1:3增加到1:2,最后增加到2:1。结果表明,随着缺氧与好氧体积比的增加,沉降性显着提高(p <0.01),同时伴随着脱氮效率的提高和丝状细菌的变化。聚合酶链反应-变性梯度凝胶电泳分析表明,细菌种类的多样性和丰富度以较小的体积比增加,但随着体积比的增加而降低,这是由于缺氧呼吸获得的能量较低。细菌以细菌为主,其次是拟杆菌。通过对应分析评估了缺氧条件对细菌群落结构的影响。就氮去除效率和沉降性而言,细菌群落结构的变化与反应器性能一致。具有不同沉降值的样品倾向于聚类为不同的组。

著录项

  • 来源
    《Desalination and water treatment》 |2015年第7期|1863-1876|共14页
  • 作者单位

    Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Shaanxi, Peoples R China;

    Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Shaanxi, Peoples R China;

    Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Shaanxi, Peoples R China;

    Xian Univ Architecture & Technol, Sch Environm & Municipal Engn, Xian 710055, Shaanxi, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Anoxic zone; Nitrogen removal; Sludge settleability; Diversity; Bacterial community;

    机译:缺氧区脱氮污泥沉降性多样性细菌群落;

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