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Effects of nitrogen source on enhancing growth conditions of green algae to produce higher lipid

机译:氮源对改善绿藻生长条件以产生更高脂质的影响

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摘要

Microalgae represent a potential source of biological material to produce biodiesel. This study focused on the effects of nitrogen concentration to enhance lipid content from Chlorella sorokiniana and their potential use to serve as a raw material for biofuel production. Light intensity and different nitrogen concentrations were used to determine the optimum cultivation environment for the fresh water microalgae, C. sorokiniana. The effects of various nitrogen sources were examined in order to determine the optimum lipid content produced by the microalgae. It was found that the optimum cultivation of microalgae growth has caused the biomass growth and has led to higher lipid production. The growth rate and lipid content were determined by measuring the optical density at 620 nm and fluorescence intensity using Nile red method. Microalgae of 10% (v/v) concentration was found to be the optimum inoculum concentration with higher growth rate obtained when compared to 50% (v/v). The NH_4NO_3 nitrogen concentration showed greater lipid production compared to NaNO_3 cells cultivated with final lipid content. The 0.2 M of NH_4NO_3 nitrogen concentration produced highest lipid (3.138 a.u), when compared to two different nitrogen sources: NH_4NO_3 and NaNO_3 with different concentrations.
机译:微藻代表生产生物柴油的生物材料的潜在来源。这项研究的重点是氮的浓度对增加Sorokiniana小球藻脂质含量的影响及其作为生物燃料生产原料的潜在用途。使用光强度和不同的氮浓度来确定淡水微藻C. sorokiniana的最佳栽培环境。为了确定微藻产生的最佳脂质含量,检查了各种氮源的影响。已经发现,微藻生长的最佳培养引起生物量的生长并导致更高的脂质产生。通过使用尼罗红法测量620nm处的光密度和荧光强度来确定生长速率和脂质含量。发现浓度为10%(v / v)的微藻是最佳接种物浓度,与50%(v / v)相比,生长速度更高。与以最终脂质含量培养的NaNO_3细胞相比,NH_4NO_3氮浓度显示出更高的脂质产生。与两个不同的氮源:不同浓度的NH_4NO_3和NaNO_3相比,0.2 M的NH_4NO_3氮浓度产生最高的脂质(3.138 a.u)。

著录项

  • 来源
    《Desalination and water treatment》 |2014年第21期|3579-3584|共6页
  • 作者单位

    Faculty of Civil Engineering, Department of Environmental Engineering, Inistitute of Environmental Water Resources and Management (IPASA), Water Research Alliance, Universiti Teknologi Malaysia, Johor 81310, Malaysia;

    Faculty of Chemical Engineering, Department of Bioprocess Engineering, Universiti Teknologi Malaysia, Johor 81310, Malaysia;

    Faculty of Civil Engineering, Department of Environmental Engineering, Inistitute of Environmental Water Resources and Management (IPASA), Water Research Alliance, Universiti Teknologi Malaysia, Johor 81310, Malaysia;

    Faculty of Civil Engineering, Department of Environmental Engineering, Inistitute of Environmental Water Resources and Management (IPASA), Water Research Alliance, Universiti Teknologi Malaysia, Johor 81310, Malaysia;

    Faculty of Bioscience and Bioengineering, Universiti Teknologi Malaysia, Johor 81310, Malaysia;

    Faculty of Bioscience and Bioengineering, Universiti Teknologi Malaysia, Johor 81310, Malaysia;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Microalgae; Chlorella sorokiniana; Nitrogen source; Lipid productivity;

    机译:微藻;小球藻氮源脂质生产力;

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