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Optical mesoscopy without the scatter: broadband multispectral optoacoustic mesoscopy

机译:无散射的光学介观:宽带多光谱光介观

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摘要

Optical mesoscopy extends the capabilities of biological visualization beyond the limited penetration depth achieved by microscopy. However, imaging of opaque organisms or tissues larger than a few hundred micrometers requires invasive tissue sectioning or chemical treatment of the specimen for clearing photon scattering, an invasive process that is regardless limited with depth. We developed previously unreported broadband optoacoustic mesoscopy as a tomographic modality to enable imaging of optical contrast through several millimeters of tissue, without the need for chemical treatment of tissues. We show that the unique combination of three-dimensional projections over a broad 500 kHz–40 MHz frequency range combined with multi-wavelength illumination is necessary to render broadband multispectral optoacoustic mesoscopy (2B-MSOM) superior to previous optical or optoacoustic mesoscopy implementations.
机译:光学介观术将生物学可视化的能力扩展到了超出显微镜所能达到的有限穿透深度的范围。但是,对大于几百微米的不透明生物或组织进行成像需要对标本进行侵入性组织切片或化学处理,以清除光子散射,而这种侵入性过程不受深度的限制。我们开发了以前从未报道过的宽带光声介观镜作为一种断层扫描方式,可以通过几毫米的组织成像光学对比,而无需对组织进行化学处理。我们表明,在500 kHz–40 MHz宽频率范围内与多个波长照明相结合的三维投影的独特组合对于使宽带多光谱光声介观镜(2B-MSOM)优于以前的光学或光声介观镜实现是必要的。

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