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首页> 外文期刊>Bangladesh Journal of Botany >Protein expression changes in leaves of bald cypress ( Taxodium distichum (L.) Rich.) in response to waterlogging
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Protein expression changes in leaves of bald cypress ( Taxodium distichum (L.) Rich.) in response to waterlogging

机译：响应于涝渍，秃柏（Taxodium distichum（L.）Rich。）叶片中的蛋白质表达变化

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摘要

To gain a better understanding of bald cypress (Taxodium distichum (L.) Rich.) response to waterlogging at the protein level, a proteomic approach has been conducted. One-year old bald cypress seedlings were selected for three waterlogging treatments (high, medium and low waterlogging), and one control (75% field capacity). Leave samples were collected at 8, 18, 38 and 60 days under waterlogging conditions. We also carried out a parallel recovery experiment by removing waterlogging at 50 days and sampled the leave ten days later. The results showed that the waterlogging treatments had significant effect on leaf soluble protein content. High and medium waterlogging increased soluble protein contents in the early stage, but decreased soluble protein content in the later stage of treatment. Low waterlogging had the highest soluble protein contents in all sampling days. The leaf protein SDS-PAGE analyses showed that three new protein subunits were found among the 28 - 34 subunits in the image profiles for the waterlogging and 10-day recovery treatments. The 2-DE leaf protein profile analysis revealed that 37 protein spots were differentially expressed, and 41 spots were shown to be up-regulated in bald cypress leaves in comparison with the control. These specific or up-regulated proteins were found to be similar with some enzymes related to aerenchyma formation, anaerobic respiration and other anaerobic polypeptides, suggesting that bald cypress had higher adaptation to waterlogging. The present study presents the evidence to further understanding the molecular response of bald cypress to waterlogging.
机译：为了更好地了解秃柏（Taxodium distichum（L.）Rich。）在蛋白质水平上对涝渍的响应，已进行了蛋白质组学研究。选择一年生的秃柏幼苗进行三种浸水处理（高，中，低浸水）和一种对照（75％的田间持水量）。在淹水条件下的第8、18、38和60天收集叶子样品。我们还通过清除50天的涝渍进行了平行恢复实验，并在10天后对假叶进行了采样。结果表明，涝渍处理对叶片可溶性蛋白含量有显着影响。高和中度渍水在早期增加可溶性蛋白质含量，但在治疗后期减少可溶性蛋白质含量。在所有采样日中，低涝渍具有最高的可溶性蛋白质含量。叶蛋白SDS-PAGE分析表明，在图像配置文件的28-34个亚基中发现了三个新的蛋白亚基，用于涝渍处理和10天恢复处理。 2-DE叶蛋白谱分析表明，与对照相比，秃柏叶中有37个蛋白点被差异表达，并且有41个点被上调。发现这些特异性或上调的蛋白质与一些与通气组织形成，厌氧呼吸和其他厌氧多肽相关的酶相似，这表明柏树对涝渍的适应性更高。本研究为进一步了解秃柏对涝渍的分子反应提供了证据。

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