• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BioResearch open access. >A Moderate Increase of Hydrogen Peroxide Level Is Beneficial for Spontaneous Resumption of Meiosis from Diplotene Arrest in Rat Oocytes Cultured In Vitro
【24h】

A Moderate Increase of Hydrogen Peroxide Level Is Beneficial for Spontaneous Resumption of Meiosis from Diplotene Arrest in Rat Oocytes Cultured In Vitro

机译：过氧化氢水平的适度增加有益于大鼠体外培养的卵母细胞自双氯芬捕捞自发恢复减数分裂。

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hydrogen peroxide (H2O2) acts as a signaling molecule and modulates various aspects of cell functions in a wide variety of cells including mammalian germ cells. We examined whether a decreased level of intra-oocyte cyclic 3′,5′-adenosine monophosphate (cAMP) leads to accumulation of H2O2, and if so, whether a moderate increase of H2O2 inactivates maturation promoting factor (MPF) during spontaneous resumption of meiosis in rat oocytes cultured in vitro . Removal of cumulus cells and culture of denuded oocytes in vitro significantly decreased oocyte cAMP level and led to spontaneous meiotic resumption from diplotene arrest. The reduced oocyte cAMP level was associated with an increased oocyte H2O2 level and reduced catalase activity. Exogenous supplementation of H2O2 induced meiotic resumption from diplotene arrest in a concentration- and time-dependent manner in oocytes treated with 0.1?mM of 3-isobutyl-1-methylxanthine, while dibutyryl-cAMP and 3- t -butyl-4-hydroxyanisole inhibited the stimulatory effect of exogenous H2O2. The increased intra-oocyte H2O2 level induced Thr-14/Tyr-15 phosphorylation of CDK1, while Thr-161 phosphorylated CDK1 and cyclin B1 levels were reduced significantly. These results suggest that a decreased level of intra-oocyte cAMP is associated with an increased level of H2O2. The increased level of H2O2 was associated with high phosphorylation of Thr-14/Tyr-15 and dephosphorylation of the Thr-161 residue of CDK1 and reduced the cyclin B1 level, which eventually inactivated MPF. The MPF inactivation triggered spontaneous resumption of meiosis from diplotene arrest in rat oocytes cultured in vitro .
机译：过氧化氢（H 2 O 2 ）充当信号分子，并调节包括哺乳动物生殖细胞在内的多种细胞的细胞功能的各个方面。我们检查了卵母细胞内环状3'，5'-单磷酸腺苷（cAMP）水平降低是否导致H 2 O 2 积累，如果是，是否在体外培养的大鼠卵母细胞自发恢复减数分裂过程中，H 2 O 2 适度增加会激活成熟促进因子（MPF）。去除卵丘细胞和体外培养裸露的卵母细胞可显着降低卵母细胞的cAMP水平，并导致自二倍体阻滞而自发减数分裂。卵母细胞cAMP水平降低与卵母细胞H 2 O 2 水平升高和过氧化氢酶活性降低有关。外源补充H 2 O 2 诱导的减数分裂由浓度浓度和时间依赖性的双戊烯阻滞在用0.1？​​mM 3-异丁基-1-处理的卵母细胞中甲基黄嘌呤，二丁酰基-cAMP和3-叔丁基-4-羟基茴香醚抑制外源H 2 O 2 的刺激作用。卵母细胞内H 2 O 2 水平升高诱导CDK1的Thr-14 / Tyr-15磷酸化，而Thr-161磷酸化的CDK1和细胞周期蛋白B1水平明显降低。这些结果表明，卵细胞内cAMP水平的降低与H 2 O 2 的水平升高有关。 H 2 O 2 的水平升高与CDK1的Thr-14 / Tyr-15的高磷酸化和Thr-161残基的去磷酸化以及细胞周期蛋白B1的降低有关级别，最终使MPF失效。 MPF失活触发自体培养的大鼠卵母细胞的二萜阻滞自发恢复减数分裂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号