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Subcutaneous Adipose Tissue–Derived Stem Cell Utility Is Independent of Anatomical Harvest Site

机译：皮下脂肪组织衍生的干细胞效用与解剖收获部位无关

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One of the challenges for tissue engineering and regenerative medicine is to obtain suitably large cell numbers for therapy. Mesenchymal stem cells (MSCs) can easily be expanded in vitro to obtain large numbers of cells, but this approach may induce cellular senescence. The characteristics of cells are dependent on variables like age, body mass index (BMI), and disease conditions, however, and in the case of adipose tissue–derived stem cells (ASCs), anatomical harvest site is also an important variable that can affect the regenerative potential of isolated cells. We therefore had kept the parameters (age, BMI, disease conditions) constant in this study to specifically assess influence of anatomical sites of individual donors on utility of ASCs. Adipose tissue was obtained from multiple anatomical sites in individual donors, and viability and nucleated cell yield were determined. MSC frequency was enumerated using colony forming unit assay and cells were characterized by flow cytometry. Growth characteristics were determined by long-term population doubling analysis of each sample. Finally, MSCs were induced to undergo adipogenic, osteogenic, and chondrogenic differentiation. To validate the findings, these results were compared with similar single harvest sites from multiple individual patients. The results of the current study indicated that MSCs obtained from multiple harvest sites in a single donor have similar morphology and phenotype. All adipose depots in a single donor exhibited similar MSC yield, viability, frequency, and growth characteristics. Equivalent differentiation capacity into osteocytes, adipocytes, and chondrocytes was also observed. On the basis of results, we conclude that it is acceptable to combine MSCs obtained from various anatomical locations in a single donor to obtain suitably large cell numbers required for therapy, avoiding in vitro senescence and lengthy and expensive in vitro culturing and expansion steps.
机译：组织工程学和再生医学的挑战之一是获得合适的大细胞数量用于治疗。间充质干细胞（MSCs）可以很容易地在体外扩增以获得大量细胞，但是这种方法可能会诱导细胞衰老。细胞的特征取决于年龄，体重指数（BMI）和疾病状况等变量，但是，对于脂肪组织衍生的干细胞（ASC），解剖收获部位也是一个重要变量，可以影响分离细胞的再生潜力。因此，在这项研究中，我们保持了参数（年龄，BMI，疾病状况）不变，以专门评估单个供体的解剖部位对ASC实用性的影响。从单个供体的多个解剖部位获得脂肪组织，并测定其生存力和有核细胞产量。使用集落形成单位测定法计数MSC频率，并通过流式细胞术表征细胞。通过对每个样品进行长期种群加倍分析来确定生长特征。最后，诱导MSC进行成脂，成骨和成软骨分化。为了验证结果，将这些结果与来自多个个体患者的相似的单个收获部位进行了比较。当前研究的结果表明，从单个供体的多个收获部位获得的MSC具有相似的形态和表型。单个供体中的所有脂肪库都表现出相似的MSC产量，生存力，频率和生长特性。还观察到了向骨细胞，脂肪细胞和软骨细胞的等效分化能力。根据结果​​，我们得出结论，在单个供体中合并从各个解剖位置获得的MSC，以获得治疗所需的适当大细胞数是可以接受的，避免了体外衰老以及冗长而昂贵的体外培养和扩增步骤。

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