• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BioResearch open access. >Enhancement of Cellular Adhesion and Proliferation in Human Mesenchymal Stromal Cells by the Direct Addition of Recombinant Collagen I Peptide to the Culture Medium
【24h】

Enhancement of Cellular Adhesion and Proliferation in Human Mesenchymal Stromal Cells by the Direct Addition of Recombinant Collagen I Peptide to the Culture Medium

机译：通过直接向培养基添加重组胶原蛋白I肽增强人间充质基质细胞的细胞粘附和增殖

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mesenchymal stromal cells (MSCs) have considerable potential for a wide range of clinical applications and regenerative medicine and cell therapy. As a consequence, there is considerable interest in developing robust culture methods for producing large number of MSCs for use in repair of injured tissues or treatment of diseases. In general, tissue culture plates or flasks that have been precoated with substrates derived from animal tissues are used in the production of MSCs. However, these substrates can potentially cause serious problems due to contamination of the MSCs with animal-derived components. In this study, we evaluated the use of a type I collagen-based recombinant peptide (RCP) for MSC culture in an attempt to avoid the problems associated with animal cell-derived substances. This RCP is xeno free, has an increased RGD (Arg–Gly–Asp) sequence, and has high molecular weight uniformity. The effect of RCP on promotion of cellular adhesion and proliferation of MSCs was investigated in cultures in which RCP was included in the culture medium. The effects of RCP on promotion of cellular adhesion and proliferation of MSCs were investigated by comparing cultures in which the additive was present in the culture medium and those where the culture plates were coated with RCP. In addition, changes in gene expression profiles during cell culture were monitored by real time-polymerase chain reaction. Our analyses showed that RCP enhanced cellular adhesion and proliferation in cultures in which the additive was included in the culture medium. Our findings indicate that adding RCP to the culture medium could save time and cost in MSC culture. Our gene expression analysis indicated that RCP enhanced expression of genes encoding proteins associated with the extracellular matrix and cell adhesion.
机译：间充质基质细胞（MSC）在广泛的临床应用以及再生医学和细胞疗法中具有巨大潜力。结果，对于开发用于产生大量MSC用于损伤组织修复或疾病治​​疗的健壮培养方法有相当大的兴趣。通常，已经用源自动物组织的底物预涂覆的组织培养板或烧瓶用于MSC的生产。但是，这些基质可能会由于动物来源的成分污染MSC而引起严重的问题。在这项研究中，我们评估了使用I型胶原基重组肽（RCP）进行MSC培养的过程，从而避免了与动物细胞源性物质相关的问题。该RCP不含异种，具有增加的RGD（Arg–Gly–Asp）序列，并且具有较高的分子量均匀性。在将RCP包含在培养基中的培养物中，研究了RCP对促进MSCs的细胞粘附和增殖的影响。通过比较在培养基中存在添加剂的培养物和用RCP包被培养板的培养物，研究了RCP对促进MSCs的细胞粘附和增殖的作用。另外，通过实时聚合酶链反应监测细胞培养期间基因表达谱的变化。我们的分析表明，RCP增强了培养基中包含添加剂的培养物中的细胞粘附和增殖。我们的发现表明，在培养基中添加RCP可以节省MSC培养的时间和成本。我们的基因表达分析表明，RCP增强了编码与细胞外基质和细胞粘附相关的蛋白质的基因的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号