Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli

German M. Traglia; Carol Davies Sala; Juan I. Fuxman Bass; Alfonso J.C. Soler-Bistué; Angeles Zorreguieta; María Soledad Ramírez; Marcelo E. Tolmasky

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Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli

机译：锁定核酸/ DNA杂合低聚物内化到大肠杆菌中

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Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAA GTACTGT TCC A CCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a stationary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.

机译：细胞内部的传递对于反义技术的实际应用至关重要。通过与Alexa Fluor 488（fLNA / DNA）偶联来标记杂合锁定核酸（LNA）/ DNA CAA GTACTGT TCC A CCA（带LNA残基的下划线），并进行测试以确定其穿透大肠杆菌细胞并到达细胞质的能力。流式细胞仪分析表明，fLNA / DNA与固定相培养中14％的细胞相关，而与标记的等序寡脱氧核苷酸的相关则可忽略不计。激光扫描共聚焦显微镜证实fLNA / DNA位于细胞质内。

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《BioResearch open access.》 |2012年第5期|共4页
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German M. Traglia; Carol Davies Sala; Juan I. Fuxman Bass; Alfonso J.C. Soler-Bistué; Angeles Zorreguieta; María Soledad Ramírez; Marcelo E. Tolmasky;
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中图分类细胞生物学;
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1. 5′-O-Methylphosphonate nucleic acids—new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes [J] . Dominik Rejman, Hana ?ípová, Ivan Barvík, Nucleic acids research . 2014,第8期

机译：5'-O-甲基膦酸核酸—新修饰的DNA，可提高大肠杆菌RNAase H杂交双链体的裂解率
2. NMR Studies of Fully Modified Locked Nucleic Acid (LNA)Hybrids:Solution Structure of an LNA:RNA Hybrid and Characterization of an LNA:DNA Hybrid [J] . Katrine E.Nielsen, Jill Rasmussen, Ravindra Kumar, Bioconjugate Chemistry . 2004,第3期

机译：完全修饰的锁核酸（LNA）杂化物的NMR研究：LNA：RNA杂种的溶液结构和LNA：DNA杂种的表征
3. DERIVATION OF NEAREST-NEIGHBOR PROPERTIES FROM DATA ON NUCLEIC ACID OLIGOMERS .2. THERMODYNAMIC PARAMETERS OF DNA-CENTER-DOT-RNA HYBRIDS AND DNA DUPLEXES [J] . Gray DM. Biopolymers: Original Research on Biomolecules and Biomolecular Assemblies . 1997,第7期

机译：从核酸低聚物的数据推导近邻性质。 DNA-CENTER-DOT-RNA杂交体和DNA双链体的热力学参数
4. Relationship between Size of Leaked Nucleic Acid of Escherichia coli and Survival Ratio by High Electric Field Pulse Application [C] . Takunao Sato, Ryo Yamashita, Daiki Yoshikawa, IEEE Conference on Electrical Insulation and Dielectric Phenomena . 2018

机译：大电场脉冲施加下大肠杆菌泄漏核酸的大小与成活率的关系
5. Interaction of the Escherichia coli single-stranded DNA-binding protein with nucleic acids and its comparison with protamine. [D] . Hasan, Shirin W. 1995

机译：大肠杆菌单链DNA结合蛋白与核酸的相互作用及其与鱼精蛋白的比较。
6. Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli [O] . German M. Traglia, Carol Davies Sala, Juan I. Fuxman Bass, 2012

机译：锁定核酸/ DNA杂合低聚物内化到大肠杆菌中
7. Infectious Nucleic Acids of Escherichia coli Bacteriophages. 10. Preparation and Properties of Escherichia coli Competent for Infectious DNA from Bacteriophages OX 174 and M 13 and RNA from Bacteriophage M 12 [O] . R. Benzinger, H. Delius, R. Jaenisch, 1967

机译：大肠杆菌噬菌体的传染性核酸。 10.大肠杆菌的制备和性质从噬菌体174和M 13和来自噬菌体M 12的菌株中的传染性DNA能力
8. Apurinic Acid Endonuclease Implicated in DNA Breakage in 'Escherichia coli' Subjected to Mild Heat. [R] . Grecz, N., Bhatarakamol, S. 1977

机译：嘌呤酸核酸内切酶与“大肠杆菌”DNa受损有关。

Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli

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