...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BioTechnologia >Anther culture response and genetic relationships between Iranian and European barley (Hordeum vulgare L.) cultivars
【24h】

Anther culture response and genetic relationships between Iranian and European barley (Hordeum vulgare L.) cultivars

机译：伊朗和欧洲大麦（Hordeum vulgare L.）品种之间的花药培养反应和遗传关系

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Barley is a widely adaptable cereal grain, but with a low response from anther cultures to callus induction. In this study, the response to anther culture and the genetic diversity of 16 Iranian and 26 European barley cultivars were evaluated using 20 ISSR (Inter Simple Sequence Repeats) primers. The callus induction phase was investigated based on a completely randomized design with an unequal number of replications. The regeneration phase was studied using a factorial experiment based on a completely randomized design with 3 replications. The factors included the tested cultivars and several growth regulators. All cultivars that responded to anther cultures (8 of 42 cultivars) had European origin. A correlation analysis showed the highest correlation between the callus size and its growth rate. A cluster analysis based on anther culture traits grouped 8 cultivars into 2 classes. The effect of growth regulators was not significant for total regeneration ratio, green and albino plants ratio. The results showed that the efficiency of the anther culture method is largely dependent on the plant genotype. The percentage of polymorphism using ISSR markers ranged from 50 to 78.94. The range of genetic similarity coefficients among barley cultivars was from 0.138 to 0.867. According to the Mantel test, there was no significant correlation between the assumed variation patterns using ISSR molecular markers and anther culture data. The cluster analysis based on the ISSR markers divided 42 barley cultivars into 6 classes, although there was no clear pattern of clustering for differentiation between Iranian and European cultivars. Iranian cultivars showed a higher molecular variation than European ones.
机译：大麦是一种适应性很强的谷物，但是花药培养对愈伤组织的诱导反应低。在这项研究中，使用20个ISSR（内部简单序列重复）引物评估了对16个伊朗大麦和26个欧洲大麦品种对花药培养的反应和遗传多样性。愈伤组织诱导期是基于完全随机的设计进行的，其重复数不相等。使用基于3次重复的完全随机设计的阶乘实验研究了再生阶段。这些因素包括测试的品种和几种生长调节剂。对花药培养有反应的所有品种（42个品种中有8个）起源于欧洲。相关分析显示愈伤组织大小与其生长速率之间的相关性最高。基于花药培养性状的聚类分析将8个品种分为2类。生长调节剂对总再生率，绿色和白化植物比例的影响并不显着。结果表明，花药培养方法的效率在很大程度上取决于植物的基因型。使用ISSR标记的多态性百分比范围为50至78.94。大麦品种之间的遗传相似性系数范围为0.138至0.867。根据Mantel测试，使用ISSR分子标记的假定变异模式与花药培养数据之间没有显着相关性。基于ISSR标记的聚类分析将42个大麦品种分为6类，尽管没有明确的聚类模式来区分伊朗品种和欧洲品种。伊朗品种的分子变异高于欧洲品种。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号