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首页> 外文期刊>Bioresources and Bioprocessing >Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba
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Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

机译：茉莉酸甲酯和水杨酸的协同作用对银杏固定化细胞培养提高银杏内酯和银杏内酯生产的影响

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Background: Bilobalide and ginkgolides are reported to be present only in Ginkgo biloba. However, only trace amounts of bilobalide and ginkgolides are contained in the ginkgo leaves. Nowadays, there has been considerable interest in plant cell cultures as a potential alternative to traditional agriculture for the industrial production of secondary metabolites. Much effort has been put into the use of in vitro cultures as one attractive biotechnological strategy for producing bilobalide and ginkgolides of commercial interest. The aim of this study was to enhance the production of bilobalide and ginkgolides A, B, and C in cell cultures of G. biloba using immobilized cell cultures and the process of elicitation. Results: Based on the effect of the immobilization components on the free cell suspension cultures, it was considered that the jute fiber acted as an elicitor and forced the cells to release the product into the culture media. The resulting biomass was approximately 1.4 times higher than in the cell suspension cultures, and the production of bilobalide and ginkgolides A, B, and C increased 5.0, 3.3, 6.1, and 4.1 times, respectively. Eliciting with methyl jasmonate (MJ) and salicylic acid (SA) in the immobilized cells enhanced bilobalide and ginkgolides A, B, and C, compared with the unelicited controls. The highest accumulation was observed using a combination of 0.1 mM MJ + 0.1 mM SA in the immobilized cells, which produced 1.78, 1.95, 2.05, and 2.95 times more bilobalide and ginkgolides A, B, and C, respectively, than the controls. Conclusions: The positive effects of immobilized cell cultures using jute fiber and the synergism effect of SA and MJ on immobilized cells of G. biloba appear to be the optimal conditions for continuous in vitro production for commercial purposes. This is the first report on analyzing the effects of jute fiber as immobilized cell material on G. biloba cell cultures and the synergism of MJ and SA on bilobalide and ginkgolide production.
机译：背景：据报道，仅在银杏中存在双叶利德和银杏内酯。但是，银杏叶中仅含有微量的银杏内酯和银杏内酯。如今，对于植物细胞培养物作为工业生产次生代谢产物的传统农业的潜在替代品，已经引起了极大的兴趣。作为生产商业利益的银杏内酯和银杏内酯的一种有吸引力的生物技术策略，已经将大量努力投入到体外培养中。这项研究的目的是利用固定化细胞培养和诱导过程，提高银杏假单胞菌细胞培养物中银杏内酯和银杏内酯A，B和C的产生。结果：基于固定化组分对游离细胞悬浮培养物的影响，认为黄麻纤维充当引发剂并迫使细胞将产物释放到培养基中。所产生的生物量比细胞悬浮培养物中的生物量高约1.4倍，银杏内酯和银杏内酯A，B和C的产量分别增加了5.0倍，3.3倍，6.1倍和4.1倍。与未诱导的对照相比，在固定化细胞中用茉莉酸甲酯（MJ）和水杨酸（SA）增强了白果内酯和银杏内酯A，B和C的活性。在固定化细胞中使用0.1 mM MJ + 0.1 mM SA的组合观察到最高积累，与对照相比，银杏内酯和银杏内酯A，B和C的产量分别高出1.78、1.95、2.05和2.95倍。结论：使用黄麻纤维固定化细胞培养物的积极作用以及SA和MJ对银杏假丝酵母固定化细胞的协同作用似乎是用于商业目的连续体外生产的最佳条件。这是关于分析黄麻纤维作为固定化细胞材料对银杏假单胞菌细胞培养物的影响以及MJ和SA对银杏内酯和银杏内酯产生的协同作用的第一份报告。

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