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首页> 外文期刊>Bali Medical Journal >Application Anti Microbial Activity Test and Direct Inoculation of Urinary Specimen Test to Increase the Quality of Results and Decrease the Production Cost in Clinical Microbiology Laboratory, Sanglah General Hospital Hospital, Bali-Indonesia
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Application Anti Microbial Activity Test and Direct Inoculation of Urinary Specimen Test to Increase the Quality of Results and Decrease the Production Cost in Clinical Microbiology Laboratory, Sanglah General Hospital Hospital, Bali-Indonesia

机译：应用抗菌活性测试和尿液标本的直接接种以提高结果质量并降低生产成本，该项目在印度尼西亚巴厘岛桑格拉总医院临床微生物学实验室进行

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Objective: Urinary tract infection (UTI) is the most common bacterial infection in general practice and in hospitals. Fast and accurate urine culture and sensitivity test are needed for adequate therapy. Anti Microbial Activity test (AMA test) that is used to detect the presence of antibiotics in urine specimens is not commonly used in clinical microbiology laboratories. Some laboratories are still using indirect inoculation technique using enriched media before inoculating onto the agar media. The aim of this research is to compare results of urinary examination of direct inoculation technique with AMA test with indirect inoculation technique without AMA test. Methods: A number of 210 urine specimens were collected in Clinical Microbiology Laboratory at Sanglah General Hospital within a time period between 16 June until 16 July 2009. Results: Antibiotics were detected in 40% of the urinary specimens; whereas 48.1% showed no evidence of UTI, that is negative AMA test and sterile urinary culture or colony growth < 105 CFU/ml. Only 11.9% of the specimens indicates urinary tract infections. The examination can be completed within 2-3 days which is shorter than indirect inoculation test which require 5-7 days. Direct inoculation technique can reduce the cost of production three-fold the costs require for an indirect inoculation test. Conclusions: Application of AMA test and direct inoculation technique can give results more rapidly, reliable and useful for clinicians. This also decrease the laboratory’s cost of production.
机译：目的：尿路感染（UTI）是一般实践和医院中最常见的细菌感染。需要进行快速，准确的尿液培养和敏感性测试，以进行适当的治疗。临床微生物学实验室通常不使用用于检测尿液标本中是否存在抗生素的抗微生物活性测试（AMA测试）。一些实验室在接种到琼脂培养基上之前仍在使用富集培养基的间接接种技术。本研究的目的是比较直接接种技术与AMA试验的尿液检查结果与间接接种技术与无AMA试验的尿液检查结果。方法：从2009年6月16日至7月16日，在Sanglah总医院临床微生物学实验室收集了210份尿液标本。结果：40％的尿液标本中检出了抗生素。而48.1％的人没有显示出UTI的证据，即AMA试验阴性和无菌尿培养或菌落生长<105 CFU / ml。仅11.9％的标本表明存在尿路感染。该检查可以在2-3天之内完成，比间接接种测试（需要5-7天）要短。直接接种技术可以将生产成本降低三倍，以进行间接接种测试。结论：AMA检测和直接接种技术的应用可为临床医生提供更快，更可靠和有用的结果。这也降低了实验室的生产成本。

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