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首页> 外文期刊>BioTechnology: An Indian Journal >Microbial community diversity of typical rivers of Changzhou, Jiangsu province
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Microbial community diversity of typical rivers of Changzhou, Jiangsu province

机译：江苏省常州市典型河流微生物群落多样性

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Microbial community structure analysis has developed on environmental science, environmental engineering, ecology and microbiology. We need to clarify the relationship between community structure and its growth environment. Based on the important role thatmicrobial community structure played in the river, we use PCR and denaturing gradient gel electrophoresis (DGGE) with primer sets targeting the V3 region of 16S rDNA genes and bacterial abundance to reflect it. The results showed that: 1) Bacterial diversities in Chaizhibang river were significantly higher than others. Bacterial communities in the tests can be classified into: Escherichia, Acinetobacter, Citrobacter, Trichococcus and Rhodobacter. However, the typical rivers had dominantly higher proportions of Escherichia and Acinetobacter. 2) The bacterial abundance has been a significant increase with the pollution, the fluctuation is from3.81 ×105 cfu/mL to 1.244 ×106 cfu/mL and average is 7.698 ×105 cfu/mL. The correlation analysis between the bacterial abundance and environmental factors reflect that CODMn and bacterial abundance had a significantly positive correlation. Next we will study the relationship betweenmicrobiology and water quality, also focus on the river water microbial communities with seasonal changes, the result provides a reference and guidance for future work.
机译：微生物群落结构分析已在环境科学，环境工程，生态学和微生物学方面得到发展。我们需要弄清社区结构与其成长环境之间的关系。基于微生物群落结构在河流中发挥的重要作用，我们使用PCR和变性梯度凝胶电泳（DGGE），其引物组针对16S rDNA基因的V3区域，并通过细菌丰度进行反映。结果表明：1）柴zhi邦河的细菌多样性明显高于其他物种。测试中的细菌群落可分为：大肠埃希氏菌，不动杆菌，柠檬酸杆菌，毛球菌和红细菌。但是，典型的河流中大肠杆菌和不动杆菌的比例较高。 2）细菌的丰度随着污染的增加而显着增加，波动范围从3.81×105 cfu / mL到1.244×106 cfu / mL，平均值为7.698×105 cfu / mL。细菌丰度与环境因素之间的相关性分析表明，CODMn与细菌丰度具有显着的正相关性。接下来，我们将研究微生物学与水质之间的关系，并着眼于季节性变化的河水微生物群落，其结果为今后的工作提供参考和指导。

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