Production of cell free-amylase by immobilized Bacillus subtilis (ATCC 6633) under the substrate stress

Jagdish Singh; Vishal Verma

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Production of cell free-amylase by immobilized Bacillus subtilis (ATCC 6633) under the substrate stress

机译：底物胁迫下固定化的枯草芽孢杆菌（ATCC 6633）产生细胞游离淀粉酶

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Living cells of Bacillus subtilis were entrapped in calcium alginate beads for the ????-amylase production. There was maximum production after 120 Hrs at pH 7 when 8???′108 cell/ml immobilized in 2% sodium alginate was inoculated in the production medium containing 5% starch and calcium chloride as inducer. Amylase extracted by acetone precipitation has specific activity 29.341 IU/mg of protein, optimumpH7 and temperature 60???°C, Km 333μgm/ml and Vmax 1.5IU/ml/minute.

机译：将枯草芽孢杆菌的活细胞捕获在藻酸钙珠粒中以产生β-淀粉酶。将pH 2固定在2％海藻酸钠中的8-8'108细胞/ ml接种到含有5％淀粉和氯化钙作为诱导剂的生产培养基中后，在pH值为7的条件下120小时后，产量最高。用丙酮沉淀法提取的淀粉酶具有29.341IU / mg蛋白质的比活，最适pH7和温度60℃，Km333μgm/ ml和Vmax 1.5IU / ml /分钟。

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《BioTechnology: An Indian Journal》 |2010年第2期|共4页
作者
Jagdish Singh; Vishal Verma;
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中图分类生物工程学（生物技术）;
关键词
?ˉ????-amylaseBacillus subtilisImmobilizationStarchsubstrate stress;

机译：α-淀粉酶-枯草芽孢杆菌固定化淀粉底物胁迫;

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1. Investigation of Cr(Ⅵ) reduction and Cr(Ⅲ) immobilization mechanism by planktonic cells and biofilms of Bacillus subtilis ATCC-6633 [J] . Xiaohong Pan, Zunjing Liu, Zhi Chen, Water Research . 2014,第maya15期

机译：枯草芽孢杆菌ATCC-6633浮游生物细胞和生物膜对Cr（Ⅵ）的还原作用及Cr（Ⅲ）的固定化机理研究
2. Crude glycerol from biodiesel industry as substrate for biosurfactant production by Bacillus subtilis ATCC 6633. [J] . Sousa M. de, Dantas I. T., Felix A. K. N., Brazilian Archives of Biology and Technology . 2014,第2期

机译：来自生物柴油行业的粗甘油，用作枯草芽孢杆菌ATCC 6633生产生物表面活性剂的底物。
3. Crude glycerol from biodiesel industry as substrate for biosurfactant production by Bacillus subtilis ATCC 6633 [J] . Anne Kamilly Nogueira Felix, Hosiberto Batista De Santana, Iuri Torquato Dantas, Brazilian Archives of Biology and Technology . 2014,第2期

机译：来自生物柴油行业的粗甘油，用作枯草芽孢杆菌ATCC 6633生产生物表面活性剂的底物
4. Immobilization of Cellulase from Bacillus subtilis UniMAP-KB01 on Multi-walled Carbon Nanotubes for Biofuel Production [C] . Sandrasekaran Naresh, Siew Hoong Shuit, Balakrishnan Kunasundari Malaysian Technical University Conference on Engineering and Technology . 2018

机译：从枯草芽孢杆菌UNIMAP-KB01固定纤维素酶对多壁碳纳米管进行生物燃料生产
5. Process optimization of alpha-amylase production by recombinant Bacillus subtilis using immobilization systems. [D] . Ramasubramanyan, Natarajan. 1994

机译：使用固定化系统优化重组枯草芽孢杆菌生产α-淀粉酶的过程。
6. Detecting Protein-Protein Interactions in the Intact Cell of Bacillus subtilis (ATCC 6633) [O] . Michael S. Winters, R. A. Day 2003

机译：在枯草芽孢杆菌完整细胞中检测蛋白质与蛋白质的相互作用（ATCC 6633）
7. Crude glycerol from biodiesel industry as substrate for biosurfactant production by Bacillus subtilis ATCC 6633 [O] . Marylane de Sousa, Iuri Torquato Dantas, Anne Kamilly Nogueira Felix, 2014

机译：来自生物柴油工业的粗甘油作为枯草芽孢杆菌aTCC 6633生物表面活性剂生产的底物
8. Physicochemical Interaction of 'Escherichia coli' Cell Envelopes and 'Bacillus subtilis' Cell Walls with Two Clays and Ability of the Composite to Immobilize Heavy Metals from Solution [R] . Walker, S. G., Flemming, C. A., Ferris, F. G., 1989

机译：“大肠杆菌”细胞包膜和“枯草芽孢杆菌”细胞壁与两种粘土的物理化学相互作用及复合物从溶液中固定重金属的能力

Production of cell free-amylase by immobilized Bacillus subtilis (ATCC 6633) under the substrate stress

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